Adaptive laboratory evolution of Escherichia coli K-12 MG1655 for growth at high hydrostatic pressure

TitleAdaptive laboratory evolution of Escherichia coli K-12 MG1655 for growth at high hydrostatic pressure
Publication TypeJournal Article
Year of Publication2015
AuthorsMarietou A, Nguyen ATT, Allen EE, Bartlett D
JournalFrontiers in Microbiology
Volume5
Date Published2015/01
Type of ArticleOriginal Research
ISBN Number1664-302X
KeywordsEscherichia coli,high pressure,piezophile,Adaptive laboratory evolution,acpP
Abstract

Much of microbial life on Earth grows and reproduces under the elevated hydrostatic pressure conditions that exist in deep-ocean and deep-subsurface environments. In this study adaptive laboratory evolution (ALE) experiments were conducted to investigate the possible modification of the piezosensitive Escherichia coli for improved growth at high pressure. After approximately 500 generations of selection, a strain was isolated that acquired the ability to grow at pressure non-permissive for the parental strain. Remarkably, this strain displayed growth properties and changes in the proportion and regulation of unsaturated fatty acids that indicated the acquisition of multiple piezotolerant properties. These changes developed concomitantly with a change in the gene encoding the acyl carrier protein, which is required for fatty acid synthesis.

DOI10.3389/fmicb.2014.00749
Short TitleE.coli growth at high pressure
Integrated Research Themes: 
Student Publication: 
No