Antarctic sea-ice microbial communities show distinct patterns of zonation in response to algal-derived substrates

TitleAntarctic sea-ice microbial communities show distinct patterns of zonation in response to algal-derived substrates
Publication TypeJournal Article
Year of Publication2014
AuthorsCowie R.OM, Williams GJ, Maas E.W, Voyles K.M, Ryan K.G
JournalAquatic Microbial Ecology
Volume73
Pagination123-134
Date Published2014/10
Type of ArticleArticle
ISBN Number0948-3055
Accession NumberWOS:000343594000003
Keywords16s ribosomal-rna; antarctica; bacteria; bacterial communities; bacterioplankton community; clone libraries; community structure; fresh-water; heterotrophic bacteria; ice core; marine waters; Metabolic function; model selection; organic-matter; phosphate; Protease; sea ice; southern-ocean
Abstract

Antarctic sea ice provides a stable environment for the colonisation of diverse and highly specialised microbes that play a central role in the assimilation and regulation of energy through the Antarctic food web. However, little is known about how the bacterial community composition changes within the sea ice, the functional role bacteria play in the sea-ice microbial loop, and the effect of variations in the environment on these patterns and processes. Using terminal restriction fragment length polymorphism (T-RFLP) analysis of the total (16S rRNA gene) and the active (16S rRNA) community, we characterised spatial patterns in Antarctic sea-ice bacterial communities (SIBCO). In addition, bacterial enzymatic activities were identified using synthetic fluorogenic substrates. Both bacterial community structure and enzymatic activity were compared to changing environmental conditions vertically down through the sea ice. The structure of the DNA-and RNA-derived bacterial communities exhibited strong vertical zonation through the ice. There was no direct relationship between changes in chlorophyll a (chl a) and bacterial numbers; however, there were direct relationships between chl a, bacterial community structure and metabolic function, thus providing evidence for a coupling of the microbial loop. The SIBCO were involved in phosphate recycling and selectively assimilated proteins over carbohydrates in response to the N- and P-poor environment. We propose that Antarctic sea ice has an active microbial loop in which the spatial dynamics of bacterial communities are influenced by algal-derived substrates and nutrient availability.

DOI10.3354/ame01710
Short TitleAquat. Microb. Ecol.
Integrated Research Themes: 
Student Publication: 
No
Research Topics: 
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