Bioassay-Guided Isolation of Antioxidant and Cytoprotective Constituents from a Maqui Berry (Aristotelia chilensis) Dietary Supplement Ingredient As Markers for Qualitative and Quantitative Analysis

TitleBioassay-Guided Isolation of Antioxidant and Cytoprotective Constituents from a Maqui Berry (Aristotelia chilensis) Dietary Supplement Ingredient As Markers for Qualitative and Quantitative Analysis
Publication TypeJournal Article
Year of Publication2017
AuthorsLi J., Yuan C.H, Pan L., Benatrehina P.A, Chai H., Keller W.J, Naman C.B, Kinghorn A.D
JournalJournal of Agricultural and Food Chemistry
Volume65
Pagination8634-8642
Date Published2017/10
Type of ArticleArticle
ISBN Number0021-8561
Accession NumberWOS:000412717300013
Keywordsanthocyanins; antioxidants; Aristotelia chilensis; cancer; chemical-constituents; chemoprevention; chilean blackberry; content determination; fruits; HPLC-DAD-ESI/MS; hydroxyl; in-vitro; maqui berry; mol. stuntz; nuclear-magnetic-resonance; oxidative stress; phloroglucinaldehyde 2-O-beta-D-glucopyranoside; quinone reductase induction; quinone reductase-inducing assay; radical-scavenging assay
Abstract

Bioassay-guided phytochemical investigation of a commercially available maqui berry (Aristotelia chilensis) extract used in botanical dietary supplement products led to the isolation of 16 compounds, including one phenolic molecule, 1, discovered for the first time from a natural source, along with several known compounds, 2-16, including three substances not reported previously in A. chilensis, 2, 14, and 15. Each isolate was characterized by detailed analysis of NMR spectroscopic and HRESIMS data and tested for their in vitro hydroxyl radical scavenging and quinone-reductase inducing biological activities. A sensitive and accurate LC-DAD-MS method for the quantitative determination of the occurrence of six bioactive compounds, 6, 7, 10-12, and 14, was developed and validated using maqui berry isolates purified in the course of this study as authentic standards. The method presented can be utilized for dereplication efforts in future natural product research projects or to evaluate chemical markers for quality assurance and batch-to-batch standardization of this botanical dietary supplement component.

DOI10.1021/acs.jafc.7b03261
Short TitleJ. Agric. Food Chem.
Student Publication: 
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