Bovine serum albumin adsorption on SiO2 and TiO2 nanoparticle surfaces at circumneutral and acidic pH: A tale of two nano-bio surface interactions

TitleBovine serum albumin adsorption on SiO2 and TiO2 nanoparticle surfaces at circumneutral and acidic pH: A tale of two nano-bio surface interactions
Publication TypeJournal Article
Year of Publication2017
AuthorsGivens B.E, Xu Z.Z, Fiegel J., Grassian VH
JournalJournal of Colloid and Interface Science
Volume493
Pagination334-341
Date Published2017/05
Type of ArticleArticle
ISBN Number0021-9797
Accession NumberWOS:000394632600037
Keywordsatr-ftir; conformational-changes; corona; in-vitro; infrared-spectroscopy; nanoparticles; protein adsorption; protein secondary structures; silica nanoparticles; structural-changes; Surface chemistry; titanium; transform ir spectroscopy
Abstract

The interaction of a model protein, bovine serum albumin (BSA) with two different metal oxide nanoparticles, TiO2 (similar to 22 nin) and SiO2 (similar to 14 nm), was studied at both physiological and acidic pH. The pH- and nanoparticle-dependent differences in protein structure and protein adsorption were determined using attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and thermogravimetric analysis (TGA). The results indicated that the surface coverage of BSA decreases with decreasing pH on both TiO2 and SiO2 surfaces, and BSA coverage is higher by a factor of ca. 3-10 times more on TiO2 compared to SiO2. The secondary structure of BSA changes upon adsorption to either nanoparticle surface at both pH 7.4 and 2. At acidic pH, BSA appears to completely unfold on TiO2 nanoparticles whereas it assumes an extended conformation on SiO2. These differences highlight for the first time the extent to which the protein corona structure is significantly impacted by protein-nanoparticle interactions which depend on the interplay between pH and specific nanoparticle surface chemistry. (C) 2017 Elsevier Inc. All rights reserved.

DOI10.1016/j.jcis.2017.01.011
Short TitleJ. Colloid Interface Sci.
Student Publication: 
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