Characterization of an orphan diterpenoid biosynthetic operon from Salinispora arenicola

TitleCharacterization of an orphan diterpenoid biosynthetic operon from Salinispora arenicola
Publication TypeJournal Article
Year of Publication2014
AuthorsXu M.M, Hillwig M.L, Lane AL, Tiernan M.S, Moore BS, Peters R.J
JournalJournal of Natural Products
Volume77
Pagination2144-2147
Date Published2014/09
Type of ArticleArticle
ISBN Number0163-3864
Accession NumberWOS:000342477900023
Keywordscloning; cyclase; ent-kaurene; gene-cluster; heterologous expression; metabolism; mevalonate pathway; mycobacterium-tuberculosis; natural-product discovery; synthases
Abstract

While more commonly associated with plants than microbes, diterpenoid natural products have been reported to have profound effects in marine microbe-microbe interactions. Intriguingly, the genome of the marine bacterium Salinispora arenicola CNS-205 contains a putative diterpenoid biosynthetic operon, terp1. Here recombinant expression studies are reported, indicating that this three-gene operon leads to the production of isopimara-8,15-dien-19-ol (4). Although 4 is not observed in pure cultures of S. arenicola, it is plausible that the terp1 operon is only expressed under certain physiologically relevant conditions such as in the presence of other marine organisms.

DOI10.1021/np500422d
Short TitleJ. Nat. Prod.
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Student Publication: 
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