Chemoecological Screening Reveals High Bioactivity in Diverse Culturable Portuguese Marine Cyanobacteria

TitleChemoecological Screening Reveals High Bioactivity in Diverse Culturable Portuguese Marine Cyanobacteria
Publication TypeJournal Article
Year of Publication2013
AuthorsLeao PN, Ramos V, Goncalves PB, Viana F, Lage OM, Gerwick WH, Vasconcelos VM
JournalMarine Drugs
Volume11
Pagination1316-1335
Date Published2013/04
Type of ArticleArticle
ISBN Number1660-3397
Accession NumberWOS:000318018700024
Keywordsbaltic sea; bioactive compounds; biological-activity; chemical ecology; cyanobacteria; identification; lake; lyngbya-majuscula; maximum-likelihood; oscillatoriales; phylogenetic analysis; screening; Secondary metabolites; sp-nov; strains
Abstract

Marine cyanobacteria, notably those from tropical regions, are a rich source of bioactive secondary metabolites. Tropical marine cyanobacteria often grow to high densities in the environment, allowing direct isolation of many secondary metabolites from field-collected material. However, in temperate environments culturing is usually required to produce enough biomass for investigations of their chemical constituents. In this work, we cultured a selection of novel and diverse cyanobacteria isolated from the Portuguese coast, and tested their organic extracts in a series of ecologically-relevant bioassays. The majority of the extracts showed activity in at least one of the bioassays, all of which were run in very small scale. Phylogenetically related isolates exhibited different activity profiles, highlighting the value of microdiversity for bioprospection studies. Furthermore, LC-MS analyses of selected active extracts suggested the presence of previously unidentified secondary metabolites. Overall, the screening strategy employed here, in which previously untapped cyanobacterial diversity was combined with multiple bioassays, proved to be a successful strategy and allowed the selection of several strains for further investigations based on their bioactivity profiles.

DOI10.3390/md11041316
Short TitleMar. Drugs
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