|Title||Chemoenzymatic synthesis of acyl coenzyme a substrates enables in situ labeling of small molecules and proteins|
|Publication Type||Journal Article|
|Year of Publication||2015|
|Authors||Agarwal V., Diethelm S., Ray L., Garg N., Awakawa T., Dorrestein PC, Moore BS|
|Type of Article||Article|
|Keywords||biosynthesis; polyketide; thioesters|
A chemoenzymatic approach to generate fully functional acyl coenzyme A molecules that are then used as substrates to drive in situ acyl transfer reactions is described. Mass spectrometry based assays to verify the identity of acyl coenzyme A enzymatic products are also illustrated. The approach is responsive to a diverse array of carboxylic acids that can be elaborated to their corresponding coenzyme A thioesters, with potential applications in wide-ranging chemical biology studies that utilize acyl coenzyme A substrates.
|Short Title||Org. Lett.|