Chemoenzymatic synthesis of acyl coenzyme a substrates enables in situ labeling of small molecules and proteins

TitleChemoenzymatic synthesis of acyl coenzyme a substrates enables in situ labeling of small molecules and proteins
Publication TypeJournal Article
Year of Publication2015
AuthorsAgarwal V., Diethelm S., Ray L., Garg N., Awakawa T., Dorrestein PC, Moore BS
JournalOrganic Letters
Volume17
Pagination4452-4455
Date Published2015/09
Type of ArticleArticle
ISBN Number1523-7060
Accession NumberWOS:000361867800014
Keywordsbiosynthesis; polyketide; thioesters
Abstract

A chemoenzymatic approach to generate fully functional acyl coenzyme A molecules that are then used as substrates to drive in situ acyl transfer reactions is described. Mass spectrometry based assays to verify the identity of acyl coenzyme A enzymatic products are also illustrated. The approach is responsive to a diverse array of carboxylic acids that can be elaborated to their corresponding coenzyme A thioesters, with potential applications in wide-ranging chemical biology studies that utilize acyl coenzyme A substrates.

DOI10.1021/acs.orglett.5b02113
Short TitleOrg. Lett.
Student Publication: 
No