Colwellia marinimaniae sp nov., a hyperpiezophilic species isolated from an amphipod within the Challenger Deep, Mariana Trench

TitleColwellia marinimaniae sp nov., a hyperpiezophilic species isolated from an amphipod within the Challenger Deep, Mariana Trench
Publication TypeJournal Article
Year of Publication2017
AuthorsKusube M., Kyaw T.S, Tanikawa K., Chastain R.A, Hardy K.M, Cameron J., Bartlett DH
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume67
Pagination824-831
Date Published2017/04
Type of ArticleArticle
ISBN Number1466-5026
Accession NumberWOS:000401992800011
KeywordsChallenger; Colwellia; deep; deep sea; depth; dna-base composition; genus; Hadal trench; japan trench; marine bacterium; meters; obligately barophilic bacterium; performance liquid-chromatography; piezophile; psychrophile; sea-ice; sediment
Abstract

An obligately piezophilic strain was isolated from an amphipod crustacean obtained in the Challenger Deep region of the Mariana Trench during the DEEPSEA CHALLENGE expedition. The strain, MTCD1(T), grew at extremely high hydrostatic pressures, with a growth range of 80-140 MPa (optimum, 120 MPa) at 6 degrees C. Phylogenetic analyses based on the 16S rRNA gene sequence indicate that it is closely affiliated with the genus Colwellia. Comparative 16S rRNA gene sequence analyses revealed 95.7, 95.5 and 95.2% similarity to Colwellia maris ABE-1(T), Colwellia piezophila Y233G(T) and Colwellia psychrerythraea ATCC 27364(T), respectively. The major cellular fatty acids were C-16 : 1, C-16 : 0 and C-22 : 6 (docosahexaenoic acid), and the sole isoprenoid quinone produced was ubiqinone-8. DNA G+C content was 48.6 mol%. The strain was positive for oxidase and catalase activities. Based on the results from this study, strain MTCD1(T) is a novel Gram-negative species of the genus Colwellia, and the name Colwellia marinimaniae sp. nov. (type strain MTCD1(T) = ATCC TSD-5(T) = JCM 30270(T)) is proposed. It is the most piezophilic organism yet described.

DOI10.1099/ijsem.0.001671
Short TitleInt. J. Syst. Evol. Microbiol.
Student Publication: 
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