Combined LC-MS/MS and molecular networking approach reveals new cyanotoxins from the 2014 cyanobacterial bloom in Green Lake, Seattle

TitleCombined LC-MS/MS and molecular networking approach reveals new cyanotoxins from the 2014 cyanobacterial bloom in Green Lake, Seattle
Publication TypeJournal Article
Year of Publication2015
AuthorsTeta R., G. Sala D, Glukhov E., Gerwick L, Gerwick WH, Mangoni A., Costantino V.
JournalEnvironmental Science & Technology
Volume49
Pagination14301-14310
Date Published2015/12
Type of ArticleArticle
ISBN Number0013-936X
Accession NumberWOS:000366872300048
Keywordsadenylation domains; anabaenopeptins; cyclic-peptides; dolastatin 10; lyngbya-majuscula; microcystis-aeruginosa; nonribosomal biosynthesis; polyketide synthases; potent; Sponge
Abstract

Cyanotoxins obtained from a freshwater cyanobacterial collection at Green Lake, Seattle during a cyanobacterial harmful algal bloom in the summer of 2014 were studied using a new approach based on molecular networking analysis of liquid chromatography tandem mass spectrometry (LC-MS/MS) data. This MS networking approach is particularly well-suited for the detection of new cyanotoxin variants and resulted in the discovery of three new cyclic peptides, namely microcystin-MhtyR (6), which comprised about half of the total microcystin content in the bloom, and ferintoic acids C (12) and D (13). Structure elucidation of 6 was aided by a new microscale methylation procedure. Metagenomic analysis of the bloom using the 16S-ITS rRNA region identified Microcystis aeruginosa as the predominant cyanobacterium in the sample. Fragments of the putative biosynthetic genes for the new cyanotoxins were also identified, and their sequences correlated to the structure of the isolated cyanotoxins.

DOI10.1021/acs.est.5b04415
Short TitleEnviron. Sci. Technol.
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