|Title||Comparative proteomics of related symbiotic mussel species reveals high variability of host-symbiont interactions|
|Publication Type||Journal Article|
|Year of Publication||2020|
|Authors||Ponnudurai R., Heiden S.E, Sayavedra L., Hinzke T., Kleiner M., Hentschker C., Felbeck H., Sievert S.M, Schluter R., Becher D., Schweder T., Markert S.|
|Type of Article||Article|
|Keywords||bacteria; bathymodiolus-azoricus; Bivalvia; carbonic-anhydrase; Environmental Sciences & Ecology; hydrogen; hydrothermal vents; metabolism; microbiology; mid-atlantic ridge; mytilidae; populations|
Deep-sea Bathymodiolus mussels and their chemoautotrophic symbionts are well-studied representatives of mutualistic host-microbe associations. However, how host-symbiont interactions vary on the molecular level between related host and symbiont species remains unclear. Therefore, we compared the host and symbiont metaproteomes of Pacific B. thermophilus, hosting a thiotrophic symbiont, and Atlantic B. azoricus, containing two symbionts, a thiotroph and a methanotroph. We identified common strategies of metabolic support between hosts and symbionts, such as the oxidation of sulfide by the host, which provides a thiosulfate reservoir for the thiotrophic symbionts, and a cycling mechanism that could supply the host with symbiont-derived amino acids. However, expression levels of these processes differed substantially between both symbioses. Backed up by genomic comparisons, our results furthermore revealed an exceptionally large repertoire of attachment-related proteins in the B. thermophilus symbiont. These findings imply that host-microbe interactions can be quite variable, even between closely related systems.