Differential transcriptional responses of carotenoid biosynthesis genes in the marine green alga Tetraselmis suecica exposed to redox and non-redox active metals

TitleDifferential transcriptional responses of carotenoid biosynthesis genes in the marine green alga Tetraselmis suecica exposed to redox and non-redox active metals
Publication TypeJournal Article
Year of Publication2019
AuthorsSathasivam R., Ki J.S
Volume46
Pagination1167-1179
Date Published2019/02
Type of ArticleArticle
ISBN Number0301-4851
Accession NumberWOS:000462022300114
Keywordsbiochemical; Biochemistry & Molecular Biology; Carotenoids; chlorophyll fluorescence; closterium-ehrenbergii; copper; dunaliella-salina; ehrenbergii meneghini conjugatophyceae; gene expression; Marine green algae; metals; microalgae; Non-redox-active; oxidative; photosystem-ii; reactive oxygen; Redox-active metals; responses; stress; Tetraselmis suecica
Abstract

The green microalga, Tetraselmis suecica, is commonly used in scientific, industrial, and aquacultural purposes because of its high stress tolerance and ease of culture in wide spectrums of environments. We hypothesized that carotenoids help to protect Tetraselmis cells from environmental stress by regulating genes in biosynthetic pathways. Here, we determined three major carotenogenic genes, phytoene synthase (PSY), phytoene desaturase (PDS), and -lycopene cyclase (LCY-B) in T. suecica, and examined the physiological parameters and gene expression responses when exposed to redox-active metals and non-redox-active metals. Phylogenetic analyses of each gene indicated that T. suecica clustered well with other green algae. Real-time PCR analysis showed that TsPSY, TsPDS, and TsLCY-B genes greatly responded to the redox-active metals in CuSO4 followed by CuCl2, but not to the non-redox-active metals. The redox-active metals strongly affected the physiology of the cells, as determined by cell counting, reactive oxygen species (ROS) imaging, and photosynthetic efficiency. This suggests that carotenoids protect the cells from oxidative damage caused by metals, thereby contributing to cell survival under various stress conditions.

DOI10.1007/s11033-018-04583-9
Student Publication: 
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