Direct capture and heterologous expression of Salinispora natural product genes for the biosynthesis of enterocin

TitleDirect capture and heterologous expression of Salinispora natural product genes for the biosynthesis of enterocin
Publication TypeJournal Article
Year of Publication2015
AuthorsBonet B., Teufel R., Crusemann M., Ziemert N., Moore BS
JournalJournal of Natural Products
Volume78
Pagination539-542
Date Published2015/03
Type of ArticleArticle
ISBN Number0163-3864
Accession NumberWOS:000352033900026
Keywordscluster; discovery; genus salinispora; marine bacterium; proteasome inhibitor; reveals; salinosporamide; secondary metabolism; streptomyces-maritimus; tropica
Abstract

Heterologous expression of secondary metabolic pathways is a promising approach for the discovery and characterization of bioactive natural products. Herein we report the first heterologous expression of a natural product from the model marine actinomycete genus Salinispora. Using the recently developed method of yeast-mediated transformation-associated recombination for natural product gene clusters, we captured a type II polyketide synthase pathway from Salinispora pacifica with high homology to the enterocin pathway from Streptomyces maritimus and successfully produced enterocin in two different Streptomyces host strains. This result paves the way for the systematic interrogation of Salinispora's promising secondary metabolome.

DOI10.1021/np500664q
Short TitleJ. Nat. Prod.
Student Publication: 
No
Research Topics: