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Diversity and antimicrobial activity of culturable fungi associated with sea anemone Anthopleura xanthogrammica

TitleDiversity and antimicrobial activity of culturable fungi associated with sea anemone Anthopleura xanthogrammica
Publication TypeJournal Article
Year of Publication2020
AuthorsLiu S, Ahmed S., Zhang C.G, Liu T.X, Shao C.L, Fang Y.W
Volume44
Pagination41-46
Date Published2020/03
Type of ArticleArticle
ISBN Number0717-3458
Accession NumberWOS:000519515900007
KeywordsAnthopleura xanthogrammica; antibacterial; Antimicrobial activity; Biotechnology & Applied Microbiology; Characterization of; diversity; Enzymatic activity; fungi; Internal transcribed spacer; Isolation of fungi; marine; Marine fungi; mucus; Sea anemone
Abstract

Background: The main objective of this study was to isolate fungi associated with Anthopleura xanthogrammica and measure their antimicrobial and enzymatic activities. A total of 93 fungal strains associated with A. xanthogrammica were isolated in this study, of which 32 isolates were identified using both morphological characteristics and internal transcribed spacer (ITS) sequence analysis. The antibacterial activities of 32 fungal isolates were tested against Bacillus subtilis, Staphylococcus aureus, Escherichia coil, Edwardsiella tarda, Vibrio harveyi, Fusarium oxysporum, and Pyricularia oryzae by agar diffusion assay. Extracellular hydrolytic enzyme activities of the fungal isolates were determined by agar diffusion assays. Enzyme activities were detected from clear halo size. Results: The isolated fungi belonged to 18 genera within 7 taxonomic orders of 1 phylum. The genera Aspergillaceae were the most diverse and common. The antimicrobial activities of 32 isolates were evaluated, and 19 (59.4%) of fungi isolate displayed unique antimicrobial activities. All fungal strains displayed at least one enzyme activity. The most common enzyme activities in the fungi isolates were amylase and protease, while the least common were pectinase and xylanase. Conclusions: This is first report on the sea anemone-derived fungi with antimicrobial and enzyme activities. Results indicated that sea anemone is a hot spot of fungal diversity and a rich resource of bioactive natural products. (C) 2020 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved.

DOI10.1016/j.ejbt.2020.01.003
Student Publication: 
No
Research Topics: 
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