Effect of closed v. intermittent-flow respirometry on hypoxia tolerance in the shiner perch Cymatogaster aggregata

TitleEffect of closed v. intermittent-flow respirometry on hypoxia tolerance in the shiner perch Cymatogaster aggregata
Publication TypeJournal Article
Year of Publication2016
AuthorsSnyder S., Nadler L.E, Bayley J.S, Svendsen M.BS, Johansen J.L, Domenici P., Steffensen J.F
JournalJournal of Fish Biology
Volume88
Pagination252-264
Date Published2016/01
Type of ArticleArticle
ISBN Number0022-1112
Accession NumberWOS:000368943000012
Keywordsaquatic breathers; bass micropterus-salmoides; cod gadus-morhua; coral-reef fishes; critical oxygen saturation level; eel; eel anguilla-anguilla; european; fresh-water fish; hypoxic; juvenile atlantic cod; oxygen-consumption; physiology; respiration; respiratory responses; stress; trout oncorhynchus-mykiss
Abstract

This study compares the critical oxygen saturation (O-2crit) levels of the shiner perch Cymatogaster aggregata obtained using two different methods wherein hypoxia is induced either by the fish's respiration (closed respirometry) or by degassing oxygen with nitrogen (intermittent-flow respirometry). Fish exhibited loss of equilibrium at a higher O-2 saturation in the closed respirometry method when compared with the intermittent-flow method. Utilization of closed respirometry yielded O-2crit measurements that were almost twice as high as those obtained with intermittent-flow respirometry. The lower hypoxia tolerance in closed respirometry is consistent with additional stress, caused by a build-up of ammonia and carbon dioxide and a faster rate in dissolved oxygen decline. The results indicate that these two methods of determining hypoxia tolerance in aquatic organisms are not comparable, and that much care should be given to method choice.

DOI10.1111/jfb.12837
Short TitleJ. Fish Biol.
Student Publication: 
Yes
Student: 
sharknado