Effects of high hydrostatic pressure on coastal bacterial community abundance and diversity

TitleEffects of high hydrostatic pressure on coastal bacterial community abundance and diversity
Publication TypeJournal Article
Year of Publication2014
AuthorsMarietou A., Bartlett DH
JournalApplied and Environmental Microbiology
Volume80
Pagination5992-6003
Date Published2014/10
Type of ArticleArticle
ISBN Number0099-2240
Accession NumberWOS:000341887100013
Keywords16s ribosomal-rna; cell-division; deep-sea; escherichia-coli; growth; in-vivo; ocean; prokaryotes; restriction-fragment-length; viable count method
Abstract

Hydrostatic pressure is an important parameter influencing the distribution of microbial life in the ocean. In this study, the response of marine bacterial populations from surface waters to pressures representative of those under deep-sea conditions was examined. Southern California coastal seawater collected 5 m below the sea surface was incubated in microcosms, using a range of temperatures (16 to 3 degrees C) and hydrostatic pressure conditions (0.1 to 80 MPa). Cell abundance decreased in response to pressure, while diversity increased. The morphology of the community also changed with pressurization to a predominant morphotype of small cocci. The pressure-induced community changes included an increase in the relative abundance of Alphaproteobacteria, Gammaproteobacteria, Actinobacteria, and Flavobacteria largely at the expense of Epsilonproteobacteria. Culturable high-pressure-surviving bacteria were obtained and found to be phylogenetically similar to isolates from cold and/or deep-sea environments. These results provide novel insights into the response of surface water bacteria to changes in hydrostatic pressure.

DOI10.1128/aem.02109-14
Integrated Research Themes: 
Student Publication: 
No
Research Topics: