The effects of pressure on pH of Tris buffer in synthetic seawater

TitleThe effects of pressure on pH of Tris buffer in synthetic seawater
Publication TypeJournal Article
Year of Publication2017
AuthorsTakeshita Y, Martz T.R, Coletti L.J, Dickson AG, Jannasch H.W, Johnson K.S
JournalMarine Chemistry
Volume188
Pagination1-5
Date Published2017/01
Type of ArticleArticle
ISBN Number0304-4203
Accession NumberWOS:000393243700001
Keywordsco2; cresol purple; dependence; High pressure; ion concentration scale; ionization; networks; ocean acidification; pacific; Partial molal volume; sea-water; seawater ph; Sensor; Tris buffer; volume
Abstract

Equimolar Tris (2-amino-2-hydroxymethyl-propane-1,3-diol) buffer prepared in artificial seawater media is a widely accepted pH standard for oceanographic pH measurements, though its change in pH over pressure is largely unknown. The change in volume (Delta V) of dissociation reactions can be used to estimate the effects of pressure on the dissociation constant of weak acid and bases. The Delta V of Tris in seawater media of salinity 35 (Delta V-Tris*) was determined between 10 and 30 degrees C using potentiometry. The potentiometric cell consisted of a modified high pressure tolerant Ion Sensitive Field Effect Transistor pH sensor and a Chloride-Ion Selective Electrode directly exposed to solution. The effects of pressure on the potentiometric cell were quantified in aqueous HCl solution prior to measurements in Tris buffer. The experimentally determined Delta V-Tris* were fitted to the equation Delta V-Tris*= 4528 +0.04912t where t is temperature in Celsius; the resultant fit agreed to experimental data within uncertainty of the measurements, which was estimated to be 0.9 cm(-3) mol(-1). Using the results presented here, change in pH of Tris buffer due to pressure can be constrained to better than 0.003 at 200 bar, and can be expressed as: DpH(Tris) = -(4.528 + 0.04912t)p/ln(10)RT. where T is temperature in Kelvin, R is the universal gas constant (83.145 cm(3) bar K-1 mol(-1)), and Pis gauge pressure in bar. On average, pH of Tris buffer changes by approximately -0.02 at 200 bar. (C) 2016 Elsevier B.V. All rights reserved.

DOI10.1016/j.marchem.2016.11.002
Student Publication: 
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