Elucidating the mode of action of marine natural products through an immunoaffinity fluorescent (iaf) approach

TitleElucidating the mode of action of marine natural products through an immunoaffinity fluorescent (iaf) approach
Publication TypeJournal Article
Year of Publication2016
AuthorsLa Clair J.J, Fenical W, Costa-Lotufo L.V
JournalJournal of the Brazilian Chemical Society
Volume27
Pagination1346-1354
Date Published2016/08
Type of ArticleArticle
ISBN Number0103-5053
Accession NumberWOS:000383789500005
Keywordsammosamides; anticancer; binding; breast-cancer; confocal microscopy; dermcidin; drug discovery; identification; Marine natural product; marinopyrrole; mcl-1; mode of action; protein; streptomyces sp; target; target elucidation
Abstract

Understanding the target and mode of action (MOA) of small molecules activity has become a critical feature in guiding the transition between drug discovery and clinical evaluation. While classically placed at the latter stages of a discovery program, we now describe how addressing the mode of action of natural products at the early stages of a program provides an important vehicle to inform the drug discovery process. In this review, we outline a streamlined cellular and molecular biological system and explore its utility through a series of four cases studies. We believe that this approach offers critical lessons to guide future drug discovery programs.

DOI10.5935/0103-5053.20160148
Short TitleJ. Braz. Chem. Soc.
Student Publication: 
No
Research Topics: 
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