Exploring the piezotolerant/piezophilic microbial community and genomic basis of piezotolerance within the deep subsurface Deccan traps

TitleExploring the piezotolerant/piezophilic microbial community and genomic basis of piezotolerance within the deep subsurface Deccan traps
Publication TypeJournal Article
Year of Publication2019
AuthorsDutta A., Peoples L.M, Gupta A., Bartlett DH, Sar P.
Volume23
Pagination421-433
Date Published2019/07
Type of ArticleArticle
ISBN Number1431-0651
Accession NumberWOS:000471018700006
Keywordsabundance; adaptation; bacteria; basalts; Biochemistry & Molecular Biology; Deccan Traps; depth; high hydrostatic-pressure; microbiology; piezophilic microbes; Piezotolerant; Pressure adaptation; quality; sea hydrothermal vents; sequences; stress; Terrestrial deep biosphere
Abstract

The deep biosphere is often characterized by multiple extreme physical-chemical conditions, of which pressure is an important parameter that influences life but remains less studied. This geomicrobiology study was designed to understand the response of a subterranean microbial community of the Deccan traps to high-pressure conditions and to elucidate their genomic properties. Groundwater from a deep basaltic aquifer of the Deccan traps was used to ascertain the community response to 25MPa and 50MPa pressure following enrichment in high-salt and low-salt organic media. Quantitative PCR data indicated a decrease in bacterial and archaeal cell numbers with increasing pressure. 16S rRNA gene sequencing displayed substantial changes in the microbial community in which Acidovorax appeared to be the most dominant genus in the low-salt medium and Microbacteriaceae emerged as the major family in the high-salt medium under both pressure conditions. Genes present in metagenome-associated genomes which have previously been associated with piezotolerance include those related to nutrient uptake and extracytoplasmic stress (omp, rseC), protein folding and unfolding (dnaK, groEL and others), and DNA repair mechanisms (mutT, uvr and others). We hypothesize that these genes facilitate tolerance to high pressure by certain groups of microbes residing in subsurface Deccan traps.

DOI10.1007/s00792-019-01094-8
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