Feeding induces translocation of vacuolar proton ATPase and pendrin to the membrane of leopard shark (Triakis semifasciata) mitochondrion-rich gill cells

TitleFeeding induces translocation of vacuolar proton ATPase and pendrin to the membrane of leopard shark (Triakis semifasciata) mitochondrion-rich gill cells
Publication TypeJournal Article
Year of Publication2014
AuthorsRoa J.N, Munevar C.L, Tresguerres M
JournalComp Biochem Physiol A Mol Integr PhysiolComp Biochem Physiol A Mol Integr Physiol
Volume174
Pagination29-37
Date Published2014/08
ISBN Number1531-4332 (Electronic)<br/>1095-6433 (Linking)
Accession Number24746982
Abstract

In this study we characterized mitochondrion-rich (MR) cells and regulation of acid/base (A/B) relevant ion-transporting proteins in leopard shark (Triakis semifasciata) gills. Immunohistochemistry revealed that leopard shark gills posses two separate cell populations that abundantly express either Na(+)/K(+)-ATPase (NKA) or V-H(+)-ATPase (VHA), but not both ATPases together. Co-immunolocalization with mitochondrial Complex IV demonstrated, for the first time in shark gills, that both NKA- and VHA-rich cells are also MR cells, and that all MR cells are either NKA- or VHA-rich cells. Additionally we localized the anion exchanger pendrin to VHA-rich cells, but not NKA-rich cells. In starved sharks, VHA was localized throughout the cell cytoplasm and pendrin was present at the apical pole (but not in the membrane). However, in a significant number of gill cells from fed leopard sharks, VHA translocated to the basolateral membrane (as previously described in dogfish), and pendrin translocated to the apical membrane. Our results highlight the importance of translocation of ion-transporting proteins to the cell membrane as a regulatory mechanism for A/B regulation.

DOI10.1016/j.cbpa.2014.04.003
Short TitleComparative biochemistry and physiology. Part A, Molecular & integrative physiologyComparative biochemistry and physiology. Part A, Molecular & integrative physiology
Alternate JournalComparative biochemistry and physiology. Part A, Molecular & integrative physiology
Integrated Research Themes: 
Student Publication: 
No