Genome sequence of the sulfur-oxidizing Bathymodiolus thermophilus gill endosymbiont

TitleGenome sequence of the sulfur-oxidizing Bathymodiolus thermophilus gill endosymbiont
Publication TypeJournal Article
Year of Publication2017
AuthorsPonnudurai R., Sayavedra L., Kleiner M., Heiden S.E, Thurmer A., Felbeck H., Schluter R., Sievert S.M, Daniel R., Schweder T., Markert S.
JournalStandards in Genomic Sciences
Volume12
Date Published2017/09
Type of ArticleArticle
ISBN Number1944-3277
Accession NumberWOS:000409355500001
KeywordsAutotrophy; deep-sea mussels; dual symbiosis; galapagos rift; hydrothermal vent mussel; hydrothermal vents; Marine invertebrate; microbial genomes; riftia-pachyptila; single-cell; sulfide-rich habitats; symbiosis; Thiotrophy; tube worm; Ultrastructure; Uncultured endosymbiont
Abstract

Bathymodiolus thermophilus, a mytilid mussel inhabiting the deep-sea hydrothermal vents of the East Pacific Rise, lives in symbiosis with chemosynthetic Gammaproteobacteria within its gills. The intracellular symbiont population synthesizes nutrients for the bivalve host using the reduced sulfur compounds emanating from the vents as energy source. As the symbiont is uncultured, comprehensive and detailed insights into its metabolism and its interactions with the host can only be obtained from culture-independent approaches such as genomics and proteomics. In this study, we report the first draft genome sequence of the sulfur-oxidizing symbiont of B. thermophilus, here tentatively named Candidatus Thioglobus thermophilus. The draft genome (3.1 Mb) harbors 3045 protein-coding genes. It revealed pathways for the use of sulfide and thiosulfate as energy sources and encodes the Calvin-Benson-Bassham cycle for CO2 fixation. Enzymes required for the synthesis of the tricarboxylic acid cycle intermediates oxaloacetate and succinate were absent, suggesting that these intermediates may be substituted by metabolites from external sources. We also detected a repertoire of genes associated with cell surface adhesion, bacteriotoxicity and phage immunity, which may perform symbiosis-specific roles in the B. thermophilus symbiosis.

DOI10.1186/s40793-017-0266-y
Short TitleStand. Genomic Sci.
Student Publication: 
No