|Title||The hybrid pyrroloisoindolone-dehydropyrrolizine alkaloid (-)-chlorizidine a targets proteins within the glycolytic pathway|
|Publication Type||Journal Article|
|Year of Publication||2015|
|Authors||Alvarez-Mico X, Rocha D.D, Guimaraes L.A, Ambrose A., Chapman E., Costa-Lotufo L.V, La Clair J.J, Fenical W|
|Type of Article||Article|
|Keywords||alpha-enolase; cancer-cells; drug discovery; elucidation; enolase; gapdh; glycolysis; mode of action; natural products|
The cytotoxic activity of (-)-chlorizidine A, a marine alkaloid containing a unique fusion between a pyrroloisoindolone and dehydropyrrolizine, was explored by using a combination of cellular and molecular methods. Our studies began by applying preliminary SAR evidence gathered from semisynthetic bioactivity evaluations to prepare an active immunoaffinity fluorescent (IAF) probe. This probe was then used to identify two cytosolic proteins, GAPDH and hENO1, as the targets of (-)chlorizidine A.