The hybrid pyrroloisoindolone-dehydropyrrolizine alkaloid (-)-chlorizidine a targets proteins within the glycolytic pathway

TitleThe hybrid pyrroloisoindolone-dehydropyrrolizine alkaloid (-)-chlorizidine a targets proteins within the glycolytic pathway
Publication TypeJournal Article
Year of Publication2015
AuthorsAlvarez-Mico X, Rocha D.D, Guimaraes L.A, Ambrose A., Chapman E., Costa-Lotufo L.V, La Clair J.J, Fenical W
JournalChembiochem
Volume16
Pagination2002-2006
Date Published2015/09
Type of ArticleArticle
ISBN Number1439-4227
Accession NumberWOS:000362815900005
Keywordsalpha-enolase; cancer-cells; drug discovery; elucidation; enolase; gapdh; glycolysis; mode of action; natural products
Abstract

The cytotoxic activity of (-)-chlorizidine A, a marine alkaloid containing a unique fusion between a pyrroloisoindolone and dehydropyrrolizine, was explored by using a combination of cellular and molecular methods. Our studies began by applying preliminary SAR evidence gathered from semisynthetic bioactivity evaluations to prepare an active immunoaffinity fluorescent (IAF) probe. This probe was then used to identify two cytosolic proteins, GAPDH and hENO1, as the targets of (-)chlorizidine A.

DOI10.1002/cbic.201500229
Short TitleChemBioChem
Student Publication: 
No
Research Topics: