Hyperspectral imaging of snow algae and green algae from aeroterrestrial habitats

TitleHyperspectral imaging of snow algae and green algae from aeroterrestrial habitats
Publication TypeJournal Article
Year of Publication2016
AuthorsHolzinger A., Allen M.C, Deheyn DD
JournalJournal of Photochemistry and Photobiology B-Biology
Volume162
Pagination412-420
Date Published2016/09
Type of ArticleArticle
ISBN Number1011-1344
Accession NumberWOS:000383003800048
Keywordsantarctic strains; Carotenoids; desiccation stress; ecophysiological performance; Green algae; high-alpine habitat; Hyperspectral imaging; phenolic-compounds; pigments; soil crust; streptophyta; Stress tolerance; Ultrastructure; uv; zygogonium-ericetorum zygnematophyceae
Abstract

Snow algae and green algae living in aeroterrestrial habitats are ideal objects to study adaptation to high light irradiation. Here, we used a detailed description of the spectral properties as a proxy for photo-acclimation/protection in snow algae (Chlamydomonas nivalis, Chlainomonas sp. and Chloromonas sp.) and charophyte green algae (Zygnema sp., Zygogonium ericetorum and Klebsormidium crenulatum). The hyperspectral microscopic mapping and imaging technique allowed us to acquire total absorption spectra of these microalgae in the waveband of 400-900 nm. Particularly in Chlamydomonas nivalis and Chlainomonas sp., a high absorbance between 400-550 nm was observed, due to naturally occurring secondary carotenoids; in Chloromonas sp. and in the charopyhte algae this high absorbance was missing, the latter being close relatives to land plants. To investigate if cellular water loss has an influence on the spectral properties, the cells were plasmolysed in sorbitol or desiccated at ambient air. While in snow algae, these treatments did hardly change the spectral properties, in the charopyhte algae the condensation of the cytoplasm and plastids increased the absorbance in the lower waveband of 400-500 nm. These changes might be ecologically relevant and photoprotective, as aeroterrestrial algae are naturally exposed to occasional water limitation, leading to desiccation, which are conditions usually occurring together with higher irradiation. (C) 2016 The Authors. Published by Elsevier B.V.

DOI10.1016/j.jphotobiol.2016.07.001
Short TitleJ. Photochem. Photobiol. B-Biol.
Student Publication: 
No
Research Topics: 
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