Coronavirus Information for the UC San Diego Community

Our leaders are working closely with federal and state officials to ensure your ongoing safety at the university. Stay up to date with the latest developments. Learn more.

The importance of a single amino acid substitution in reduced red blood cell carbonic anhydrase function of early-diverging fish

TitleThe importance of a single amino acid substitution in reduced red blood cell carbonic anhydrase function of early-diverging fish
Publication TypeJournal Article
Year of Publication2020
AuthorsDichiera A.M, McMillan O.JL, Clifford A.M, Goss G.G, Brauner C.J, Esbaugh A.J
Volume190
Pagination287-296
Date Published2020/05
Type of ArticleArticle
ISBN Number0174-1578
Accession NumberWOS:000526824900003
KeywordsCarbonic anhydrase; catalytic; Chondrichthyans; co2; dioxide transport; dogfish; enhance oxygen delivery; erythrocytes; evolution; Gas exchange; hagfish; hemoglobin-function; lamprey lampetra-fluviatilis; mechanism; physiology; Red blood cell; Root effect; vertebrate; zoology
Abstract

In most vertebrates, red blood cell carbonic anhydrase (RBC CA) plays a critical role in carbon dioxide (CO2) transport and excretion across epithelial tissues. Many early-diverging fishes (e.g., hagfish and chondrichthyans) are unique in possessing plasma-accessible membrane-bound CA-IV in the gills, allowing some CO2 excretion to occur without involvement from the RBCs. However, implications of this on RBC CA function are unclear. Through homology cloning techniques, we identified the putative protein sequences for RBC CA from nine early-diverging species. In all cases, these sequences contained a modification of the proton shuttle residue His-64, and activity measurements from three early-diverging fish demonstrated significantly reduced CA activity. Site-directed mutagenesis was used to restore the His-64 proton shuttle, which significantly increased RBC CA activity, clearly illustrating the functional significance of His-64 in fish red blood cell CA activity. Bayesian analyses of 55 vertebrate cytoplasmic CA isozymes suggested that independent evolutionary events led to the modification of His-64 and thus reduced CA activity in hagfish and chondrichthyans. Additionally, in early-diverging fish that possess branchial CA-IV, there is an absence of His-64 in RBC CAs and the absence of the Root effect [where a reduction in pH reduces hemoglobin's capacity to bind with oxygen (O-2)]. Taken together, these data indicate that low-activity RBC CA may be present in all fish with branchial CA-IV, and that the high-activity RBC CA seen in most teleosts may have evolved in conjunction with enhanced hemoglobin pH sensitivity.

DOI10.1007/s00360-020-01270-9
Student Publication: 
No