Integrating mass spectrometry and genomics for cyanobacterial metabolite discovery

TitleIntegrating mass spectrometry and genomics for cyanobacterial metabolite discovery
Publication TypeJournal Article
Year of Publication2016
AuthorsMoss N.A, Bertin M.J, Kleigrewe K., Leao T.F, Gerwick L, Gerwick WH
JournalJournal of Industrial Microbiology & Biotechnology
Volume43
Pagination313-324
Date Published2016/03
Type of ArticleArticle
ISBN Number1367-5435
Accession NumberWOS:000372538000020
Keywordsbiosynthesis; biosynthesis gene-cluster; cyanobacteria; cyanobacterium; cyclic depsipeptides; genomics; heterologous production; lyngbya-majuscula; marine; mass spectrometry; molecular networking; natural products; natural-product; polyketide synthase; secondary metabolite; synthase docking domains
Abstract

Filamentous marine cyanobacteria produce bioactive natural products with both potential therapeutic value and capacity to be harmful to human health. Genome sequencing has revealed that cyanobacteria have the capacity to produce many more secondary metabolites than have been characterized. The biosynthetic pathways that encode cyanobacterial natural products are mostly uncharacterized, and lack of cyanobacterial genetic tools has largely prevented their heterologous expression. Hence, a combination of cutting edge and traditional techniques has been required to elucidate their secondary metabolite biosynthetic pathways. Here, we review the discovery and refined biochemical understanding of the olefin synthase and fatty acid ACP reductase/aldehyde deformylating oxygenase pathways to hydrocarbons, and the curacin A, jamaicamide A, lyngbyabellin, columbamide, and a trans-acyltransferase macrolactone pathway encoding phormidolide. We integrate into this discussion the use of genomics, mass spectrometric networking, biochemical characterization, and isolation and structure elucidation techniques.

DOI10.1007/s10295-015-1705-7
Short TitleJ. Ind. Microbiol. Biotechnol.
Student Publication: 
Yes
Student: 
sharknado