Isolation and structure elucidation of lipopeptide antibiotic taromycin B from the activated taromycin biosynthetic gene cluster

TitleIsolation and structure elucidation of lipopeptide antibiotic taromycin B from the activated taromycin biosynthetic gene cluster
Publication TypeJournal Article
Year of Publication2018
AuthorsReynolds K.A, Luhavaya H., Li J., Dahesh S., Nizet V., Yamanaka K, Moore BS
JournalJournal of Antibiotics
Volume71
Pagination333-338
Date Published2018/02
Type of ArticleArticle
ISBN Number0021-8820
Accession NumberWOS:000424117000019
Keywordsa21978c; amphomycin; Biotechnology & Applied Microbiology; complex; daptomycin; Immunology; insights; microbiology; Pharmacology & Pharmacy
Abstract

In the ongoing effort to unlock the chemical potential of marine bacteria, genetic engineering of biosynthetic gene clusters (BGCs) is increasingly used to awake or improve expression of biosynthetic genes that may lead to discovery of novel bioactive natural products. Previously, we reported the successful capture, engineering and heterologous expression of an orphan BGC from the marine actinomycete Saccharomonospora sp. CNQ-490, which resulted in the isolation of the novel lipopeptide antibiotic taromycin A. Herein we report the isolation and structure elucidation of taromycin B, the second most abundant product of the taromycin biosynthetic series, and show that taromycins A and B exhibit complex chromatographic properties indicative of interconverting conformations. Taromycins A and B display potent activity against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecium clinical isolates, suggestive that the taromycin molecular scaffold is a promising starting point for further derivatization to produce compounds with promising antibiotic characteristics.

DOI10.1038/ja.2017.146
Short TitleJ. Antibiot.
Student Publication: 
No