Isolation of diverse amoebal grazers of freshwater cyanobacteria for the development of model systems to study predator-prey interactions

TitleIsolation of diverse amoebal grazers of freshwater cyanobacteria for the development of model systems to study predator-prey interactions
Publication TypeJournal Article
Year of Publication2016
AuthorsMa A.T, Daniels E.F, Gulizia N., Brahamsha B
JournalAlgal Research-Biomass Biofuels and Bioproducts
Volume13
Pagination85-93
Date Published2016/01
Type of ArticleArticle
ISBN Number2211-9264
Accession NumberWOS:000370183100009
Keywordsacanthamoeba-castellanii; Algae; algal biofuels; amebas; Amoeba; biofuel production; Blue-green algae; classification; Crop; cyanobacteria; eukaryotic diversity; Grazers; identification; microalgae; parasites; phylogeny; Protection
Abstract

A common method for large-scale production of algal crops is growth in outdoor open-air ponds. While this approach is more cost-effective, outdoor open-air ponds are prone to contamination by competing algae, pathogens, and eukaryotic grazers, including ciliates, flagellates, and amoebae. To characterize grazers of cyanobacteria, or blue-green algae, we have performed enrichments and isolations from water samples obtained from environmental sites and from an experimental production pond. We obtained a set of amoebal isolates that show diversity in phylogeny, morphology, and locomotion. After examination of grazing on solid medium and in liquid medium, we found that some amoebal isolates can graze on a range of cyanobacterial species, while other amoebal isolates appear to have a more limited prey range. These prey ranges correlate with observed growth rates and cyst formation, suggesting differing growth and survival strategies for amoebae in the environment. Taken together, this work provides a glimpse into the range of natural amoebal predators of cyanobacteria and establishes model systems of predator-prey interactions. Further characterization of these systems will facilitate development of strategies for crop protection of open-air algal production ponds. (C) 2015 Elsevier B.V. All rights reserved.

DOI10.1016/j.algal.2015.11.010
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