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Linkage of marine bacteria polyunsaturated fatty acid and long-chain hydrocarbon biosynthesis

TitleLinkage of marine bacteria polyunsaturated fatty acid and long-chain hydrocarbon biosynthesis
Publication TypeJournal Article
Year of Publication2019
AuthorsAllemann M.N, Shulse C.N, Allen EE
JournalFrontiers in Microbiology
Date Published2019/04
Type of ArticleArticle
ISBN Number1664-302X
Accession NumberWOS:000463394100001
Keywordsbroad host range; expression; gene; genome sequence; high-pressure; hydrocarbon; identification; microbiology; mutagenesis; omega-3 polyunsaturated fatty acid; Photobacterium; photobacterium-profundum ss9; Shewanella; thioesterase; to-head hydrocarbon; vectors

Vaious marine gamma-proteobacteria produce omega-3 polyunsaturated fatty acids, such as eicosapentaenoic acid (20:5, EPA) and docosahexaenoic acid (22:6, DHA), which are incorporated into membrane phospholipids. Five genes, designated pfaABCDE, encode the polyketide/fatty acid synthase necessary for production of these long-chain fatty acids. In addition to de novo biosynthesis of EPA and DHA, the "Pfa synthase" is also involved with production of a long-chain polyunsaturated hydrocarbon product (31:9, PUHC) in conjunction with the oleABCD hydrocarbon biosynthesis pathway. In this work, we demonstrate that OleA mediates the linkage between these two pathways in vivo. Co-expression of pfaA-E along with oleA from Shewanella pealeana in Escherichia coli yielded the expected product, a 31:8 ketone along with a dramatic similar to 10-fold reduction in EPA content. The decrease in EPA content was independent of 31:8 ketone production as co-expression of an OleA active site mutant also led to identical decreases in EPA content. We also demonstrate that a gene linked with either pfa and/or ole operons in diverse bacterial lineages, herein designated pfaT, plays a role in maintaining optimal production of Pfa synthase derived products in Photobacterium and Shewanella species.

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