Coronavirus Information for the UC San Diego Community

Our leaders are working closely with federal and state officials to ensure your ongoing safety at the university. Stay up to date with the latest developments. Learn more.

Linkage of marine bacterial polyunsaturated fatty acid and long-chain hydrocarbon biosynthesis

TitleLinkage of marine bacterial polyunsaturated fatty acid and long-chain hydrocarbon biosynthesis
Publication TypeJournal Article
Year of Publication2019
AuthorsAllemann MN, Shulse CN, Allen EE
JournalFrontiers in Microbiology
Date Published2019/04
Type of ArticleOriginal Research
ISBN Number1664-302X
KeywordsOmega - 3 fatty acids,hydrocarbon,Thioesterase,Shewanella,Photobacterium

Various marine gamma-proteobacteria produce omega-3 polyunsaturated fatty acids, such as eicosapentaenoic acid (20:5, EPA) and docosahexaenoic acid (22:6, DHA), which are incorporated into membrane phospholipids. Five genes, designated pfaABCDE, encode the polyketide/fatty acid synthase necessary for production of these long-chain fatty acids. In addition to de novo biosynthesis of EPA and DHA, the “Pfa synthase” is also involved with production of a long-chain polyunsaturated hydrocarbon product (31:9, PUHC) in conjunction with the oleABCD hydrocarbon biosynthesis pathway. In this work, we demonstrate that OleA mediates the linkage between these two pathways in vivo. Co-expression of pfaA-E along with oleA from Shewanella pealeana in Escherichia coli yielded the expected product, a 31:8 ketone along with a dramatic ~10-fold reduction in EPA content. The decrease in EPA content was independent of 31:8 ketone production as co-expression of an OleA active site mutant also led to identical decreases in EPA content. We also demonstrate that a gene linked with either pfa and/or ole operons in diverse bacterial lineages, herein designated pfaT, plays an accessory role in maintaining optimal production of Pfa synthase derived products in Photobacterium and Shewanella species.

Short TitleLong chain polyunsaturated bacterial products
Student Publication: 
Research Topics: