Marine Actinobacteria from the Gulf of California: diversity, abundance and secondary metabolite biosynthetic potential

TitleMarine Actinobacteria from the Gulf of California: diversity, abundance and secondary metabolite biosynthetic potential
Publication TypeJournal Article
Year of Publication2013
AuthorsBecerril-Espinosa A, Freel KC, Jensen PR, Soria-Mercado IE
JournalAntonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
Volume103
Pagination809-819
Date Published2013/04
Type of ArticleArticle
ISBN Number0003-6072
Accession NumberWOS:000316145900012
KeywordsActinobacteria; actinomycete salinispora; bacteria; enzymatic domains; gen. nov.; Gulf of California; natural-product; polyketide synthase; Polyketide synthetase (PKS); saccharopolyspora-erythraea; Salinispora; Secondary metabolites; sediments; south china sea; sp-nov.; Streptomyces
Abstract

The Gulf of California is a coastal marine ecosystem characterized as having abundant biological resources and a high level of endemism. In this work we report the isolation and characterization of Actinobacteria from different sites in the western Gulf of California. We collected 126 sediment samples and isolated on average 3.1-38.3 Actinobacterial strains from each sample. Phylogenetic analysis of 136 strains identified them as members of the genera Actinomadura, Micromonospora, Nocardiopsis, Nonomuraea, Saccharomonospora, Salinispora, Streptomyces and Verrucosispora. These strains were grouped into 26-56 operational taxonomic units (OTUs) based on 16S rRNA gene sequence identities of 98-100 %. At 98 % sequence identity, three OTUs appear to represent new taxa while nine (35 %) have only been reported from marine environments. Sixty-three strains required seawater for growth. These fell into two OTUs at the 98 % identity level and include one that failed to produce aerial hyphae and was only distantly related (a parts per thousand currency sign95.5 % 16S identity) to any previously cultured Streptomyces sp. Phylogenetic analyses of ketosynthase domains associated with polyketide synthase genes revealed sequences that ranged from 55 to 99 % nucleotide identity to experimentally characterized biosynthetic pathways suggesting that some may be associated with the production of new secondary metabolites. These results indicate that marine sediments from the Gulf of California harbor diverse Actinobacterial taxa with the potential to produce new secondary metabolites.

DOI10.1007/s10482-012-9863-3
Short TitleAntonie Van Leeuwenhoek
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