The metabolic potential of the single cell genomes obtained from the Challenger Deep, Mariana Trench within the candidate superphylum Parcubacteria (OD1)

DEEPSEA CHALLENGER on the seafloor, imaged from the accompanying lander
TitleThe metabolic potential of the single cell genomes obtained from the Challenger Deep, Mariana Trench within the candidate superphylum Parcubacteria (OD1)
Publication TypeJournal Article
Year of Publication2017
AuthorsLeon-Zayas R., Peoples L., Biddle J.F, Podell S, Novotny M., Cameron J., Lasken R.S, Bartlett DH
JournalEnvironmental Microbiology
Volume19
Pagination2769-2784
Date Published2017/07
Type of ArticleArticle
ISBN Number1462-2912
Accession NumberWOS:000405907300020
Keywordsbacterial diversity; division od1; escherichia-coli; high-throughput; insights; multiple sequence alignment; physiology; prokaryotes; proteins; sediments
Abstract

Candidate phyla (CP) are broad phylogenetic clusters of organisms that lack cultured representatives. Included in this fraction is the candidate Parcubacteria superphylum. Specific characteristics that have been ascribed to the Parcubacteria include reduced genome size, limited metabolic potential and exclusive reliance on fermentation for energy acquisition. The study of new environmental niches, such as the marine versus terrestrial subsurface, often expands the understanding of the genetic potential of taxonomic groups. For this reason, we analyzed 12 Parcubacteria single amplified genomes (SAGs) from sediment samples collected within the Challenger Deep of the Mariana Trench, obtained during the Deepsea Challenge (DSC) Expedition. Many of these SAGs are closely related to environmental sequences obtained from deep-sea environments based on 16S rRNA gene similarity and BLAST matches to predicted proteins. DSC SAGs encode features not previously identified in Parcubacteria obtained from other habitats. These include adaptation to oxidative stress, polysaccharide modification and genes associated with respiratory nitrate reduction. The DSC SAGs are also distinguished by relative greater abundance of genes for nucleotide and amino acid biosynthesis, repair of alkylated DNA and the synthesis of mechanosensitive ion channels. These results present an expanded view of the Parcubacteria, among members residing in an ultra-deep hadal environment.

DOI10.1111/1462-2920.13789
Impact: 

Parcubacteria reside in diverse habitats, including deepā€sea sediments. Here we report on Parcubacteria within the microbial population present in the surficial sediments of the Challenger Deep, Mariana Trench. The genome analyses presented here reinforce the view of the Parcubacteria as organisms with small genomes that are able to metabolize organics by fermentation. While considered to have a small genomic size, they also appear capable of expanding their genome. The significance of the newly identified features to the lifestyle and environmental contributions of this enigmatic but abundant super phylum awaits further investigation.

Student Publication: 
Yes
Student: 
sharknado