Metabolic profiling directly from the petri dish using nanospray desorption electrospray ionization imaging mass spectrometry

TitleMetabolic profiling directly from the petri dish using nanospray desorption electrospray ionization imaging mass spectrometry
Publication TypeJournal Article
Year of Publication2013
AuthorsWatrous J., Roach P., Heath B., Alexandrov T, Laskin J, Dorrestein PC
JournalAnalytical Chemistry
Volume85
Pagination10385-10391
Date Published2013/11
Type of ArticleArticle
ISBN Number0003-2700
Accession NumberWOS:000326711400059
Keywordsbacillus-subtilis; bacteria; cytochromes; exchange; genome; identification; shewanella-oneidensis mr-1; streptomyces-coelicolor
Abstract

Understanding molecular interaction pathways in complex biological systems constitutes a treasure trove of knowledge that might facilitate the specific, chemical manipulation of the countless microbiological systems that occur throughout our world. However, there is a lack of methodologies that allow the direct investigation of chemical gradients and interactions in living biological systems, in real time. Here, we report the use of nanospray desorption electrospray ionization (nanoDESI) imaging mass spectrometry for in vivo metabolic profiling of living bacterial colonies directly from the Petri dish with absolutely no sample preparation needed. Using this technique, we investigated single colonies of Shewanella oneidensis MR-1, Bacillus subtilis 3610, and Streptomyces coelicolor A3(2) as well as a mixed biofilm of S. oneidensis MR-1 and B. subtilis 3610. Data from B. subtilis 3610 and S. coelicolor A3(2) provided a means of validation for the method while data from S. oneidensis MR-1 and the mixed biofilm showed a wide range of compounds that this bacterium uses for the dissimilatory reduction of extracellular metal oxides, including riboflavin, iron-bound heme and heme biosynthetic intermediates, and the siderophore putrebactin.

DOI10.1021/ac4023154
Short TitleAnal. Chem.
Student Publication: 
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