Metagenomic discovery of polybrominated diphenyl ether biosynthesis by marine sponges

TitleMetagenomic discovery of polybrominated diphenyl ether biosynthesis by marine sponges
Publication TypeJournal Article
Year of Publication2017
AuthorsAgarwal V., Blanton J.M, Podell S, Taton A., Schorn M.A, Busch J., Lin Z.J, Schmidt EW, Jensen PR, Paul VJ, Biggs J.S, Golden J.W, Allen EE, Moore BS
JournalNature Chemical Biology
Volume13
Pagination537-+
Date Published2017/05
Type of ArticleArticle
ISBN Number1552-4450
Accession NumberWOS:000399004000015
Keywords4; cyanobacterium oscillatoria-spongeliae; dibenzo-p-dioxins; dictyoceratid sponges; dysidea-herbacea; lamellodysidea; lissoclinum-patella; metabolites; natural-products; organic-compounds; secondary; symbiont
Abstract

Naturally produced polybrominated diphenyl ethers (PBDEs) pervade the marine environment and structurally resemble toxic man-made brominated flame retardants. PBDEs bioaccumulate in marine animals and are likely transferred to the human food chain. However, the biogenic basis for PBDE production in one of their most prolific sources, marine sponges of the order Dysideidae, remains unidentified. Here, we report the discovery of PBDE biosynthetic gene clusters within sponge-microbiome-associated cyanobacterial endosymbionts through the use of an unbiased metagenome-mining approach. Using expression of PBDE biosynthetic genes in heterologous cyanobacterial hosts, we correlate the structural diversity of naturally produced PBDEs to modifications within PBDE biosynthetic gene clusters in multiple sponge holobionts. Our results establish the genetic and molecular foundation for the production of PBDEs in one of the most abundant natural sources of these molecules, further setting the stage for a metagenomic-based inventory of other PBDE sources in the marine environment.

DOI10.1038/nchembio.2330
Short TitleNat. Chem. Biol.
Student Publication: 
Yes
Student: 
sharknado