Mooreia alkaloidigena gen. nov., sp nov and Catalinimonas alkaloidigena gen. nov., sp nov., alkaloid-producing marine bacteria in the proposed families Mooreiaceae fam. nov and Catalimonadaceae fam. nov in the phylum Bacteroidetes

TitleMooreia alkaloidigena gen. nov., sp nov and Catalinimonas alkaloidigena gen. nov., sp nov., alkaloid-producing marine bacteria in the proposed families Mooreiaceae fam. nov and Catalimonadaceae fam. nov in the phylum Bacteroidetes
Publication TypeJournal Article
Year of Publication2013
AuthorsChoi E.J, Beatty D.S, Paul L.A, Fenical W, Jensen PR
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume63
Pagination1219-1228
Date Published2013/04
Type of ArticleArticle
ISBN Number1466-5026
Accession NumberWOS:000319785700002
Keywordsactinomycete; alignment; diversity; emended description; gliding bacterium; ocean; rapidithrix-thailandica; roseivirga; samples; sea
Abstract

Bacterial strains CNX-216(T) and CNU-914(T) were isolated from marine sediment samples collected from Palmyra Atoll and off Catalina Island, respectively. Both strains were Gram-negative and aerobic and produce deep-orange to pink colonies and alkaloid secondary metabolites. Cells of strain CNX-216(T) were short, non-motile rods, whereas cells of strain CNU-914(T) were short, curved rods with gliding motility. The DNA G + C contents of CNX-216(T) and CNU-914(T) were respectively 57.7 and 44.4 mol%. Strains CNX-216(T) and CNU-914(T) contained MK-7 as the predominant menaquinone and iso-C-15:0 and C-(16:1)omega 5c as the major fatty acids. Phylogenetic analyses revealed that both strains belong to the order Cytophagales in the phylum Bacteroicletes. Strain CNX-216(T) exhibited low 16S rRNA gene sequence identity (87.1 %) to the nearest type strain, Cesiribacter roseus 311(T), and formed a well-supported lineage that is outside all currently described families in the order Cytophagales. Strain CNU-914(T) shared 97.6% 16S rRNA gene sequence identity with 'Porifericola rhodea' N5EA6-3A2B and, together with 'Tunicatimonas pelagia' N5DB8-4 and four uncharacterized marine bacteria isolated as part of this study, formed a lineage that is clearly distinguished from other families in the order Cytophagales. Based on our polyphasic taxonomic characterization, we propose that strains CNX-216(T) and CNU-914(T) represent novel genera and species, for which we propose the names Mooreia alkaloidigena gen. nov., sp. nov. (type strain CNX-216(T) =DSM 25187(T) =KCCM 90102(T)) and Catalinimonas alkaloidigena gen. nov., sp. nov. (type strain CNU-914(T) =DSM 25186(T) =KCCM 90101(T)) within the new families Mooreiaceae fam. nov. and Catalimonadaceae fam. nov.

DOI10.1099/ijs.0.043752-0
Short TitleInt. J. Syst. Evol. Microbiol.
Integrated Research Themes: