Nutrient stoichiometry shapes microbial community structure in an evaporitic shallow pond

TitleNutrient stoichiometry shapes microbial community structure in an evaporitic shallow pond
Publication TypeJournal Article
Year of Publication2017
AuthorsLee Z.MP, Poret-Peterson A.T, Siefert J.L, Kaul D., Moustafa A., Allen A.E, Dupont C.L, Eguiarte L.E, Souza V., Elser J.J
JournalFrontiers in Microbiology
Volume8
Date Published2017/05
Type of ArticleArticle
ISBN Number1664-302X
Accession NumberWOS:000402245000001
KeywordsAlgae; Availability; bacteria; bacterioplankton; beta diversity; cienegas; community structure; cuatro; diversity; ecology; fresh-water bacteria; genes; grazing snails; growth rate hypothesis; operon copy number; phosphorus enrichment; rRNA gene copy number; stoichiometry
Abstract

Nutrient availability and ratios can play an important role in shaping microbial communities of freshwater ecosystems. The Cuatro Cienegas Basin (CCB) in Mexico is a desert oasis where, perhaps paradoxically, high microbial diversity coincides with extreme oligotrophy. To better understand the effects of nutrients on microbial communities in CCB, a mesocosm experiment was implemented in a stoichiometrically imbalanced pond, Lagunita, which has an average TN:TP ratio of 122 (atomic). The experiment had four treatments, each with five spatial replicates - unamended controls and three fertilization treatments with different nitrogen: phosphorus (N:P) regimes (P only, N:P = 16 and N:P = 75 by atoms). In the water column, quantitative PCR of the 16S rRNA gene indicated that P enrichment alone favored proliferation of bacterial taxa with high rRNA gene copy number, consistent with a previously hypothesized but untested connection between rRNA gene copy number and P requirement. Bacterial and microbial eukaryotic community structure was investigated by pyrosequencing of 16S and 18S rRNA genes from the planktonic and surficial sediment samples. Nutrient enrichment shifted the composition of the planktonic community in a treatment-specific manner and promoted the growth of previously rare bacterial taxa at the expense of the more abundant, potentially endemic, taxa. The eukaryotic community was highly enriched with phototrophic populations in the fertilized treatment. The sediment microbial community exhibited high beta diversity among replicates within treatments, which obscured any changes due to fertilization. Overall, these results showed that nutrient stoichiometry can be an important factor in shaping microbial community structure.

DOI10.3389/fmicb.2017.00949
Student Publication: 
No