PCR-independent method of transformation-associated recombination reveals the cosmomycin biosynthetic gene cluster in an ocean streptomycete

TitlePCR-independent method of transformation-associated recombination reveals the cosmomycin biosynthetic gene cluster in an ocean streptomycete
Publication TypeJournal Article
Year of Publication2017
AuthorsLarson C.B, Crusemann M., Moore BS
JournalJournal of Natural Products
Volume80
Pagination1200-1204
Date Published2017/04
Type of ArticleArticle
ISBN Number0163-3864
Accession NumberWOS:000400538800055
Keywordsanthracycline; cloning; expression; mass-spectrometry; natural-products
Abstract

The transformation-associated recombination cloning methodology facilitates the genomic capture and heterologous expression of natural product biosynthetic gene clusters (BGCs). We have streamlined this procedure by introduction of synthetic DNA gene blocks for the efficient capture of BGCs. We show the successful capture and expression of the aromatic polyketide antitumor agent cosmomycin from streptomycete bacteria and the discovery of new cosmomycin analogues by mass spectral molecular networking.

DOI10.1021/acs.jnatprod.6b01121
Short TitleJ. Nat. Prod.
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