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A peptidyl-transesterifying type I thioesterase in salinamide biosynthesis

TitleA peptidyl-transesterifying type I thioesterase in salinamide biosynthesis
Publication TypeJournal Article
Year of Publication2016
AuthorsRay L., Yamanaka K, Moore BS
JournalAngewandte Chemie-International Edition
Date Published2016/01
Type of ArticleArticle
ISBN Number1433-7851
Accession NumberWOS:000368065300053
Keywordsadenylation; antibiotics; antiinflammatory depsipeptides; biosynthesis; bond formation; cloning; domains; gene clusters; intermolecular transesterification; marine streptomycete; mechanisms; peptides; salinamide; thioesterase

Salinamide A belongs to a rare class of bicyclic depsipeptide antibiotics in which the installation of a (4-methylhexa-2,4-dienoyl) glycine handle across a hexadepsipeptide core contributes to its chemical complexity and biological properties. Herein, we report the genetic and biochemical basis for salinamide construction in the marine bacterium Streptomyces sp. CNB-091, which involves a novel intermolecular transesterification reaction catalyzed by a type I thioesterase. Heterologous expression studies revealed the central role of the nonribosomal peptide synthetase Sln9 in constructing and installing the distinctive acylglycine "basket handle" of salinamide. Biochemical characterization of the Sln9 thioesterase domain established that transesterification of the serine residue of desmethylsalinamide E with acylated glycyl thioesters yields desmethylsalinamide C.

Short TitleAngew. Chem.-Int. Edit.
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