Preparative separation and purification of trichothecene mycotoxins from the marine fungus Fusarium sp LS68 by high-speed countercurrent chromatography in stepwise elution mode

TitlePreparative separation and purification of trichothecene mycotoxins from the marine fungus Fusarium sp LS68 by high-speed countercurrent chromatography in stepwise elution mode
Publication TypeJournal Article
Year of Publication2018
AuthorsLiu Y., Zhou X.Z, Naman C.B, Lu Y.B, Ding L.J, He S.
JournalMarine Drugs
Volume16
Date Published2018/02
Type of ArticleArticle
ISBN Number1660-3397
Accession NumberWOS:000427528800035
Keywordsbiosynthesis; chromatography; graminearum; head blight; high-speed countercurrent; macrocyclic trichothecenes; metabolites; Pharmacology & Pharmacy; phytotoxicity; preparative separation; risk; roridin; stepwise elution; trichoderma; trichothecene mycotoxins; verrucarin; wheat
Abstract

The contamination of foods and animal feeds with trichothecene mycotoxins is a growing concern for human and animal health. As such, large quantities of pure trichothecene mycotoxins are necessary for food safety monitoring and toxicological research. A new and effective method for the purification of trichothecene mycotoxins from a marine fungus, Fusarium sp. LS68, is described herein. Preparative high-speed countercurrent chromatography (HSCCC) was utilized for the scalable isolation and purification of four trichothecene mycotoxins for the first time in stepwise elution mode, with a biphasic solvent system composed of hexanes-EtOAc-CH3OH-H2O (6:4:5:5, v/v/v/v) and (8.5:1.5:5:5,v/v/v/v). This preparative HSCCC separation was performed on 200 mg of crude sample to yield four trichothecene mycotoxins, roridin E (1), roridin E acetate (2), verrucarin L acetate (3), and verrucarin J (4) in a single run, with each of >98% purity. These compounds were identified by MS, H-1 NMR, C-13 NMR, and polarimetry. The results demonstrate an efficient HSCCC method for the separation of trichothecene mycotoxins, which can be utilized to produce pure commercial and research standards.

DOI10.3390/md16020073
Short TitleMar. Drugs
Student Publication: 
No
Research Topics: 
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