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Previously uncultured marine bacteria linked to novel alkaloid production

TitlePreviously uncultured marine bacteria linked to novel alkaloid production
Publication TypeJournal Article
Year of Publication2015
AuthorsChoi E.J, Nam SJ, Paul L., Beatty D., Kauffman CA, Jensen PR, Fenical W
JournalChemistry & Biology
Date Published2015/09
Type of ArticleArticle
ISBN Number1074-5521
Accession NumberWOS:000364012100012
Keywordsantitumor antibiotics; chloropeptin-i; gliding bacterium; in-situ cultivation; pure culture; soil bacteria; sp-nov.; staphylococcus-aureus; streptomyces sp; structure elucidation

Low-nutrient media and long incubation times facilitated the cultivation of 20 taxonomically diverse Gram-negative marine bacteria within the phyla Bacteroidetes and Proteobacteria. These strains comprise as many as three new families and include members of clades that had only been observed using culture-independent techniques. Chemical studies of the type strains representing two new families within the order Cytophagales led to the isolation of nine new alkaloid secondary metabolites that can be grouped into four distinct structure classes, including azepinones, aziridines, quinolones, and pyrazinones. Several of these compounds possess antibacterial properties and appear, on structural grounds, to be produced by amino acid-based biosynthetic pathways. Our results demonstrate that relatively simple cultivation techniques can lead to the isolation of new bacterial taxa that are capable of the production of alkaloid secondary metabolites with antibacterial activities. These findings support continued investment in cultivation techniques as a method for natural product discovery.

Short TitleChem. Biol.
Student Publication: 
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