Role of TRP Channels in dinoflagellate mechanotransduction

TitleRole of TRP Channels in dinoflagellate mechanotransduction
Publication TypeJournal Article
Year of Publication2017
AuthorsLindstrom J.B, Pierce N.T, Latz M.I
JournalBiological Bulletin
Volume233
Pagination151-167
Date Published2017/10
Type of ArticleArticle
ISBN Number0006-3185
Accession NumberWOS:000423262700005
Keywordsactivated; arachidonic-acid; bioluminescence; calcium-channels; cation channels; gonyaulax-polyedra; ion-channel; Life Sciences & Biomedicine - Other Topics; lingulodinium-polyedrum; Marine & Freshwater Biology; mechanosensitive channels; multiple sequence alignment; polyunsaturated fatty-acids; stimulated
Abstract

Transient receptor potential (TRP) ion channels are common components of mechanosensing pathways, mainly described in mammals and other multicellular organisms. To gain insight into the evolutionary origins of eukaryotic mechanosensory proteins, we investigated the involvement of TRP channels in mechanosensing in a unicellular eukaryotic protist, the dinoflagellate Lingulodiniumpolyedra. BLASTPanalysis of the protein sequences predicted from the L. polyedra transcriptome revealed six sequences with high similarity to human TRPM2, TRPM8, TRPML2, TRPP1, and TRPP2; and characteristic TRP domains were identified in all sequences. In a phylogenetic tree including all mammalian TRP subfamilies and TRP channel sequences from unicellular and multicellular organisms, the L. polyedra sequences grouped with the TRPM, TPPML, and TRPP clades. In pharmacological experiments, we used the intrinsic bioluminescence of L. polyedra as a reporter of mechanoresponsivity. Capsaicin and RN1734, agonists of mammalian TRPV, and arachidonic acid, an agonist of mammalian TRPV, TRPA, TRPM, and Drosophila TRP, all stimulated bioluminescence in L. polyedra. Mechanical stimulation of bioluminescence, but not capsaicinstimulated bioluminescence, was inhibited by gadolinium (Gd3+), a general inhibitor of mechanosensitive ion channels, and the phospholipase C (PLC) inhibitor U73122. These pharmacological results are consistent with the involvement of TRP-like channels in mechanosensing by L. polyedra. The TRP channels do not appear to be mechanoreceptors but rather are components of the mechanotransduction signaling pathway and may be activated via a PLC-dependent mechanism. The presence and function of TRP channels in a dinoflagellate emphasize the evolutionary conservation of both the channel structures and their functions.

DOI10.1086/695421
Short TitleBiol. Bull.
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