Single dish gradient screening of small molecule localization

TitleSingle dish gradient screening of small molecule localization
Publication TypeJournal Article
Year of Publication2016
AuthorsBeuzer P., Axelrod J., Trzoss L., Fenical W, Dasari R., Evidente A., Kornienko A., Cang H., La Clair J.J
JournalOrganic & Biomolecular Chemistry
Date Published2016/09
Type of ArticleArticle
ISBN Number1477-0520
Accession NumberWOS:000383932900006
Keywordscalmodulin; fluorescent-probes; live-cell; migration; natural-products; ophiobolin-a; organic fluorophores; superresolution microscopy; target elucidation; tracking

Understanding trafficking in cells and tissues is one of the most critical steps in exploring the mechanisms and modes of action (MOAs) of a small molecule. Typically, deciphering the role of concentration presents one of the most difficult challenges associated with this task. Herein, we present a practical solution to this problem by developing concentration gradients within single dishes of cells. We demonstrate the method by evaluating fluorescently-labelled probes developed from two classes of natural products that have been identified as potential anti-cancer leads by STORM super-resolution microscopy.

Short TitleOrg. Biomol. Chem.
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