Submarine basaltic glass colonization by the heterotrophic Fe(II)-Oxidizing and siderophore-producing deep-sea bacterium Pseudomonas stutzeri VS-10: The potential role of basalt in enhancing growth

TitleSubmarine basaltic glass colonization by the heterotrophic Fe(II)-Oxidizing and siderophore-producing deep-sea bacterium Pseudomonas stutzeri VS-10: The potential role of basalt in enhancing growth
Publication TypeJournal Article
Year of Publication2017
AuthorsSudek L.A, Wanger G., Templeton A.S, Staudigel H., Tebo BM
JournalFrontiers in Microbiology
Volume8
Date Published2017/03
Type of ArticleArticle
ISBN Number1664-302X
Accession NumberWOS:000395870100001
KeywordsBasalt; biofilm; biomass; diffusion chamber; electron-transfer; extracellular; Fe(II) oxidation; floor basalt; hydrothermal plumes; iron-oxidizing bacteria; loihi seamount; marine basalts; microbial fuel cell; microbial fuel-cells; ocean; production; shewanella-oneidensis mr-1; siderophores
Abstract

Phylogenetically and metabolically diverse bacterial communities have been found in association with submarine basaltic glass surfaces. The driving forces behind basalt colonization are for the most part unknown. It remains ambiguous if basalt provides ecological advantages beyond representing a substrate for surface colonization, such as supplying nutrients and/or energy. Pseudomonas stutzeri VS-10, a metabolically versatile bacterium isolated from Vailulu'u Seamount, was used as a model organism to investigate the physiological responses observed when biofilms are established on basaltic glasses. In Fe-limited heterotrophic media, P. stutzeri VS-10 exhibited elevated growth in the presence of basaltic glass. Diffusion chamber experiments demonstrated that physical attachment or contact of soluble metabolites such as siderophores with the basaltic glass plays a pivotal role in this process. Electrochemical data indicated that P. stutzeri VS-10 is able to use solid substrates (electrodes) as terminal electron donors and acceptors. Siderophore production and heterotrophic Fe(II) oxidation are discussed as potential mechanisms enhancing growth of P. stutzeri VS-10 on glass surfaces. In correlation with that we discuss the possibility that metabolic versatility could represent a common and beneficial physiological trait in marine microbial communities being subject to oligotrophic and rapidly changing deep-sea conditions.

DOI10.3389/fmicb.2017.00363
Student Publication: 
No