A Tandem Chemoenzymatic Methylation by S-Adenosyl-L-methionine

TitleA Tandem Chemoenzymatic Methylation by S-Adenosyl-L-methionine
Publication TypeJournal Article
Year of Publication2013
AuthorsLipson JM, Thomsen M, Moore BS, Clausen RP, La Clair JJ, Burkart MD
JournalChembiochem
Volume14
Pagination950-953
Date Published2013/05
ISBN Number1439-7633
Keywordsalkylation; chemoenzymatic synthesis; DNA methylation; methyltransferase; natural products
Abstract

Keep ′em methylated: The in situ preparation of the cofactor AdoMet was achieved by allowing the biosynthetic enzyme SalL to operate in the reverse direction by presentation of 5′-chloro-5′-deoxyadenosine at low salt concentrations. This reaction was readily coupled with DNA and small molecule methyltransferases to afford a regioselective method for chemo-enzymatic methylation and isotope incorporation.

DOI10.1002/cbic.201300221
Short TitleChemBioChem
Integrated Research Themes: 
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