|Title||A Tandem Chemoenzymatic Methylation by S-Adenosyl-L-methionine|
|Publication Type||Journal Article|
|Year of Publication||2013|
|Authors||Lipson JM, Thomsen M, Moore BS, Clausen RP, La Clair JJ, Burkart MD|
|Keywords||alkylation; chemoenzymatic synthesis; DNA methylation; methyltransferase; natural products|
Keep ′em methylated: The in situ preparation of the cofactor AdoMet was achieved by allowing the biosynthetic enzyme SalL to operate in the reverse direction by presentation of 5′-chloro-5′-deoxyadenosine at low salt concentrations. This reaction was readily coupled with DNA and small molecule methyltransferases to afford a regioselective method for chemo-enzymatic methylation and isotope incorporation.