A thin soup: extraction and amplification of DNA from DMSO and ethanol used as preservative for cetacean tissue samples

TitleA thin soup: extraction and amplification of DNA from DMSO and ethanol used as preservative for cetacean tissue samples
Publication TypeJournal Article
Year of Publication2013
AuthorsRobertson KM, Minich J., Bowman A.J, Morin PA
JournalConservation Genetics Resources
Volume5
Pagination929-933
Date Published2013/12
Type of ArticleArticle
ISBN Number1877-7252
Accession NumberWOS:000326387800009
KeywordsDMSO; DNA extraction; ETOH; Mitochondrial; mtDNA; nDNA; pcr; Preservative; specimens
Abstract

Two popular tissue preservatives, 100% ethanol and 20% salt saturated dimethyl sulfoxide (DMSO) solution were tested for the existence of amplifiable, free-floating DNA after 2-18 years of tissue storage. We found that short mtDNA fragments were consistently amplified and sequenced from DMSO preservative, while nDNA amplification was limited and inconsistent. Amplification of both mtDNA and nDNA failed most of the time for the ethanol samples.

DOI10.1007/s12686-013-9934-4
Short TitleConserv. Genet. Resour.
Integrated Research Themes: 
Student Publication: 
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