A two-column flash chromatography approach to pyoverdin production from Pseudomonas putida GB1

TitleA two-column flash chromatography approach to pyoverdin production from Pseudomonas putida GB1
Publication TypeJournal Article
Year of Publication2017
AuthorsDuckworth O.W, Markarian D.S, Parker D.L, Harrington J.M
JournalJournal of Microbiological Methods
Volume135
Pagination11-13
Date Published2017/04
Type of ArticleArticle
ISBN Number0167-7012
Accession NumberWOS:000397547600003
Keywordsbacterium; Flash chromatography; fluorescens; purification; roles; siderophores
Abstract

Our knowledge of the biological and environmental reactivity of siderophores is limited by the difficulty and cost of obtaining reasonable quantities by purification or synthesis. In this note, we describe a modified procedure for the low-cost, mg-scale purification of pyoverdin-type siderophores using a dual-flash chromatography (reverse phase absorption and size exclusion) approach. (C) 2017 Elsevier B.V. All rights reserved.

DOI10.1016/j.mimet.2017.01.019
Short TitleJ. Microbiol. Methods
Student Publication: 
No
Research Topics: 
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