Viscous control of cellular respiration by membrane lipid composition

TitleViscous control of cellular respiration by membrane lipid composition
Publication TypeJournal Article
Year of Publication2018
AuthorsBudin I., de Rond T., Chen Y., Chan L.JG, Petzold C.J, Keasling J.D
Date Published2018/12
Type of ArticleArticle
ISBN Number0036-8075
Accession NumberWOS:000452506300062
Keywordsbiological-membranes; electron-transfer; escherichia-coli; evidence; homeoviscous adaptation; inner-membrane; kinetic; lateral diffusion-coefficients; nadh dehydrogenase; phospholipid-vesicles; Science & Technology - Other Topics; terminal oxidases

Lipid composition determines the physical properties of biological membranes and can vary substantially between and within organisms. We describe a specific role for the viscosity of energy-transducing membranes in cellular respiration. Engineering of fatty acid biosynthesis in Escherichia coil allowed us to titrate inner membrane viscosity across a 10-fold range by controlling the abundance of unsaturated or branched lipids. These fluidizing lipids tightly controlled respiratory metabolism, an effect that can be explained with a quantitative model of the electron transport chain (ETC) that features diffusion-coupled reactions between enzymes and electron carriers (quinones). Lipid unsaturation also modulated mitochondrial respiration in engineered budding yeast strains. Thus, diffusion in the ETC may serve as an evolutionary constraint for lipid composition in respiratory membranes.

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