Cryo-Electron Microscope Facility

Facility Name: 
Cryo-Electron Microscope Facility
Chemistry & Biochemistry

The Cryo-Electron Microscopy Facility is available to UCSD faculty, staff and students and also to members of the non-profit research institutions on the Torrey Pines Mesa. The FEI Tecnai G2 Polara is a 300KV transmission electron microscope (TEM) equipped with a field emission gun (FEG). It has a newly developed stage that allows for examination of multiple, vitrified samples at either liquid nitrogen or liquid helium temperatures as well as a state of the art K2 direct detector and energy filter. The FEI Tecnai G2 Sphera is a 200KV TEM equipped with a LaB6 electron gun. It has the standard holder for dry samples and two Gatan cryotransfer holders. The Polara is equipped with the SerialEM tomography software and the Leginon image acquisition system. Both microscopes are available for use on a recharge basis, however use of the Polara will be restricted to those scientists whose projects require the higher resolution afforded by the FEG or the greater stability of the Compustage. The only sample preparation techniques performed in the facility are plunge freezing of particulate samples for cryomicroscopy and negative staining. Operation of the instruments by Facility staff is available as is hands-on training on the instruments.

Timothy Baker
TEM (transmission electron microscope)
Director Email: