DATE: Monday June 13th
LOCATION: 10:30am Hubbs Hall 4500
The anaerobic oxidation of methane accounts for the removal of ~80% of methane produced below the seafloor. In marine methane seeps, AOM is predominantly catalyzed by syntrophic archaeal-bacterial consortia. Due to their slow in situ growth rates, our understanding of the factors determining niche-partitioning between different consortia is limited, and other microbial associations may await their discovery.
I have recently developed a novel approach, bioorthogonal non-canonical amino acid tagging (BONCAT), for the in situ tracking of protein synthesis in uncultured microbes. By combining BONCAT-staining with other molecular methods (such as FISH and FACS), we were able to track thousands of individual, biosynthetically active cell partnerships – orders of magnitude more than would have been possible using traditional, isotope-labeling approaches.
In my talk, I will discuss the advantages and limitations of BONCAT for studying uncultured microbes in complex samples, present how our recent studies have provided us with exciting new insights into microbial interactions in deep-sea sediment, and what steps are to be taken next if we want to understand biogeochemical cycling and cellular interactions in environmental and human host samples.
Faculty Host: Kit Pogliano (email@example.com) x21314