Shared Facilities

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VC Area / SIO Section Department Facility Description Instruments
BIO CMBB CMBB Shared Instruments

500 MHz NMRs (one newly purchased in 2016) and high resolution LC/MS instrumentation. Other equipment is shared among the organic chemistry oriented groups such as FT-IR, polarimeter and fermentation facilities, 2 additional Low Resolution LCMS instruments, 1 GCMS, many HPLCs (probably around 10 total), 1 FPLC, Teledyne Isco CombiFlash Rf+ Lumen with UV and ELS detectors


Director: Bill Gerwick
Contact:
NMR (Nuclear Magnetic Resonance), LC/MS (Liquid Chromatography-Mass Spectrometry), FT-IR (Fourier-Transform Infrared Spectroscopy), Polarimeter and fermentation facilities, GCMS (Gas Chromatography Mass Spectrometry), HPLC (High Performance Liquid Chromatography), FPLC (Fast Protein Liquid Chromatography), CombiFlash Rf+ Lumen with UV and ELS detectors
BIO MBRD Experimental Aquarium

Facilities for in vivo studies include two large experimental aquarium rooms. Hubbs Hall experimental aquarium facility is 2751 square feet with 12 trays and 30 tanks (most insulated) of volumes ranging from 1020 l to 7560 l, utilizing an 'open seawater system' that can deliver chilled (8 deg C), ambient (12 deg C-21 deg C), and warm (28 deg C) seawater . Chilled and warm seawater temperatures are very reliable, usually fluctuating no more then +/- 1 C deg. Four additional aquarium wet labs exist throughout Hubbs Hall, including a chilled 'closed system' in a controlled temperature room. The Ritter Hall Experimental Aquarium facility is 2150 square feet with 18 trays and 19 tanks with volumes ranging from 300 l to 21,000 l, a two chambered controlled light room and three each 3 m square cubicle rooms. Chilled (10 deg C) and ambient (12 deg C-21 deg C) seawater is also available via an 'open system'.


Director: Mark Hildebrand
Contact: Phil Zerofski
BIO MBRD Genomic Instrumentation

Much of this equipment is heavily used and nearing the end of its useful life: Stratagene Mx3000P real-time PCR machine; EC fluorescence and chemiluminescence imager); Kodak CCD-based imager for gel documentation andrefrigerated centrifuge; Molecular Devices M2 microplate reader; Amersham Typhoon 8600 laser-based multi-mode imager (phosphoimager, fluorescence and chemiluminescence imager); Kodak CCD-based imager for gel documentation and chemiluminescence applications; Perkin Elmer Lamda 35 UV-Vis spectrophotometer; NanoDrop spectrophotometer.


Director: R. Burton
Contact:
PCR machine (polymerase chain reaction), EC Fluorescence and Chemiluminescence Imager, Imager (CCD-based), Centrifuge (refrigerated), Molecular Devices M2 Microplate Reader, Multi-mode Imager, Laser-based Multi-mode Imager (phosphoimager, fluorescence and chemiluminescence), Spectrophotometer
BIO MBRD Cell Population Analysis Tools for Oceanography and Biotechnology

Cell Population Analysis System (Influx sorting flow cytometer; ImageStreamX Imaging Cytometer). Use reserved for SIO only.


Use of the Influx flow cytometer requires training at the Becton Dickenson training facility for one week prior to use.  Please arrange for completion of this training (http://www.bdbiosciences.com/us/s/training/classroombased) prior to
anticipated use.

Director: Mark Hildebrand/Brian Palenik
Contact: Mark Hildebrand
Cytometer (influx sorting flow), Cytometer (imaging )
BIO MBRD Molecular Biology Instrumentation

BD Influx Sorting Flow Cytometer
Amnis ImageStream X Mark II Imaging Flow Cytometer
Beckman Coulter Multisizer 4 Coulter Counter
Luminex 100 Suspension Array System
Roche Lightcycler and Stratagene Mx3000P real-time PCR machines
BioRad 2-D Electophoresis System
BioRad Chef-DR III Variable Angle Electrophoresis System
Molecular Devices M2 Microplate Reader (for absorbance and fluorescence, with excitation and emission monochrometers)
Perkin Elmer Lambda 35 UV-Vis Spectrophotometer with 7 cuvette positioner and temp control
NanoDrop ND-1000 UV-Vis Spectrophotometer
Amersham Typhoon 9410 Laser-based Multi-mode Imager (phosphoimager, fluorescence and chemiluminescence imager)
BioRad GelDoc XR+ Imaging System
Alpha-Innotech ChemiImager 5500 (CCD-based imager for gel documentation and chemiluminescence applications)
IEC refrigerated centrifuge with microplate rotor
Beckman L8-70M Ultracentrifuge with rotors
SLM Aminco French Pressure Cell Press.


Some equipment requires special training and/or providing necessary reagents/supplies.  Contact for use cost.

Director: Eric Allen/Mark Hildebrand
Contact: Eric Allen
Flow Cytometer, Cytometer (Imaging Flow), Coulter Counter, Suspension Array System, Lightcycler, PCR (polymerase chain reaction) machines real-time, Electophoresis System, Electrophoresis System (Variable Angle), Molecular Devices M2 Microplate Reader, XR+ Imaging System, Centrifuge (refrigerated), Pressure Cell Press, Imager (CCD-based)
EARTH GRD

XRF (X-ray fluorescence) instrument available for shared use.  Training required and includes:

(1) discussion of the physics of XRF in an easy-to-understand manner, (2) teach you to apply knowledge of XRF to elemental analysis of anything, (3) allow you to analyze YOUR (solid) samples, and (4) hands-on access to Tracer XRF instruments.  


Director: Jane Willenbring
Contact:
XRF (X-ray fluorescence)
EARTH GRD Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES) Facility

Perkin Elmer Optima 3000 DV ICP-OES (Inductively coupled plasma - optical emission spectrometer). This instrument provides fast and reliable analysis of major and minor elements of small samples in solution. Used for routine multi-element analysis (ppb to ppm range) of environmental, geological or biological samples.


Director: Pat Castillo
Contact: Chris Macisaac
ICP-OES (Inductively Coupled Plasma Optical Emission Spectrometer)
EARTH GRD Inductively Coupled Plasma Mass Spectrometer (ICP-MS) Facility

The facility houses two Thermo Fisher Scientific™ iCAP™ Qc ICP-MS instruments. One is dedicated to performing the analysis of samples in solution, the other to performing direct analysis of solid samples by laser ablation. Application types include but are not limited to biological, environmental and geological analyses. ICP-MS instruments can rapidly measure almost the entire Periodic Table simultaneously at a wide range of concentrations (sub ppb to ppm). The facility has the capability to prepare certain sample solutions if required. Both solution and laser instruments are available on a recharge basis.

Additionally the facility has a ThermoQuest Finnigan Element 2™ HR-ICP-MS which has the ability to measure isotope ratios and a Thermo Fisher Scientific™ Triton Plus™ TIMS for the highest precision measurements. These instruments are used to address the needs of SIO users.
Visit the website (http://sigl.ucsd.edu/) if you would like to learn more about the facility or enquire about access to the instruments.


Cost:

Solution ICP-MS - $9 measurement

Laser Ablation ICP-MS - $14 measurement

Solution Sample Preparation - $91 per hour

Data Analysis - $80 per hour

We can provide sample tubes, solutions etc., for an additional cost

Director: James Day
Contact: Ruan Hattingh
ICP-MS (Inductively Coupled Plasma Mass Spectrometer), TIMS (Thermal Ionization Mass Spectrometry)
EARTH GRD High-Precision Mass Spectrometers - Noble Gas Isotopes as Environmental Tracers

High-precision mass spectrometers for noble gas isotopes as environmental tracers. 2 ea Finnigan MAT 253 Isotope Ratio Mass Spectrometers


Shared informally with students, postdocs, faculty

Director: Jeff Severinghaus
Contact: Jeff Severinghaus
Mass Spectrometer (isotope ratio)
EARTH GRD Rock Sample Preparation Facility

Facility include rock saws, crushing devices (ceramic and metal) and two shatter boxes for the preparation of crushed rock material for mineral separation and for the preparation of rock powders. These facilities, along with mineral separation (Franz magnetic separators) and polishing equipment, are available to qualified users.


Director: James Day
Contact: Megan Smith
Rock saws, Crushing devices (ceramic and metal), Shatter boxes, Magnetic separators (Franz), Rock polishing equipment