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|CMBB Shared Instruments||
500 MHz NMRs (one newly purchased in 2016) and high resolution LC/MS instrumentation. Other equipment is shared among the organic chemistry oriented groups such as FT-IR, polarimeter and fermentation facilities, 2 additional Low Resolution LCMS instruments, 1 GCMS, many HPLCs (probably around 10 total), 1 FPLC, Teledyne Isco CombiFlash Rf+ Lumen with UV and ELS detectors.
Facilities for in vivo studies include two large experimental aquarium rooms. Hubbs Hall experimental aquarium facility is 2751 square feet with 12 trays and 30 tanks (most insulated) of volumes ranging from 1020 l to 7560 l, utilizing an 'open seawater system' that can deliver chilled (8 deg C), ambient (12 deg C-21 deg C), and warm (28 deg C) seawater . Chilled and warm seawater temperatures are very reliable, usually fluctuating no more then +/- 1 C deg. Four additional aquarium wet labs exist throughout Hubbs Hall, including a chilled 'closed system' in a controlled temperature room. The Ritter Hall Experimental Aquarium facility is 2150 square feet with 18 trays and 19 tanks with volumes ranging from 300 l to 21,000 l, a two chambered controlled light room and three each 3 m square cubicle rooms. Chilled (10 deg C) and ambient (12 deg C-21 deg C) seawater is also available via an 'open system'.
Type: Experimental AQ
Much of this equipment is heavily used and nearing the end of its useful life: Stratagene Mx3000P real-time PCR machine; EC fluorescence and chemiluminescence imager); Kodak CCD-based imager for gel documentation andrefrigerated centrifuge; Molecular Devices M2 microplate reader; Amersham Typhoon 8600 laser-based multi-mode imager (phosphoimager, fluorescence and chemiluminescence imager); Kodak CCD-based imager for gel documentation and chemiluminescence applications; Perkin Elmer Lamda 35 UV-Vis spectrophotometer; NanoDrop spectrophotometer.
|Cell Population Analysis Tools for Oceanography and Biotechnology||
Cell Population Analysis System (Influx sorting flow cytometer; ImageStreamX Imaging Cytometer). Use reserved for SIO only.
Use of the Influx flow cytometer requires training at the Becton Dickenson training facility for one week prior to use. Please arrange for completion of this training (http://www.bdbiosciences.com/us/s/training/classroombased) prior to
|Molecular Biology Instrumentation||
BD Influx Sorting Flow Cytometer
Some equipment requires special training and/or providing necessary reagents/supplies. Contact for use cost.
|Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES) Facility||
Perkin Elmer Optima 3000 DV ICP-OES (Inductively coupled plasma - optical emission spectrometer). This instrument provides fast and reliable analysis of major and minor elements of small samples in solution. Used for routine multi-element analysis (ppb to ppm range) of environmental, geological or biological samples.
|Inductively Coupled Plasma Mass Spectrometer (ICP-MS) Facility||
The facility houses two Thermo Fisher Scientific™ iCAP™ Qc ICP-MS instruments. One is dedicated to performing the analysis of samples in solution, the other to performing direct analysis of solid samples by laser ablation. Application types include but are not limited to biological, environmental and geological analyses. ICP-MS instruments can rapidly measure almost the entire Periodic Table simultaneously at a wide range of concentrations (sub ppb to ppm). The facility has the capability to prepare certain sample solutions if required. Both solution and laser instruments are available on a recharge basis.
Additionally the facility has a ThermoQuest Finnigan Element 2™ HR-ICP-MS which has the ability to measure isotope ratios and a Thermo Fisher Scientific™ Triton Plus™ TIMS for the highest precision measurements. These instruments are used to address the needs of SIO users.
Solution ICP-MS - $9 measurement
Laser Ablation ICP-MS - $14 measurement
Solution Sample Preparation - $91 per hour
Data Analysis - $80 per hour
We can provide sample tubes, solutions etc., for an additional cost
|High-Precision Mass Spectrometers - Noble Gas Isotopes as Environmental Tracers||
High-precision mass spectrometers for noble gas isotopes as environmental tracers. 2 ea Finnigan MAT 253 Isotope Ratio Mass Spectrometers
Shared informally with students, postdocs, faculty
|Rock Sample Preparation Facility||
Facility include rock saws, crushing devices (ceramic and metal) and two shatter boxes for the preparation of crushed rock material for mineral separation and for the preparation of rock powders. These facilities, along with mineral separation (Franz magnetic separators) and polishing equipment, are available to qualified users.