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|VC Area / SIO Section||Department||Facility||Description||Instruments|
|CAMPUS||Calit2||Nano3 Nanofabrication Cleanroom Facility||
Nano3 offers advanced capabilities for fabrication and characterization of devices at the micro- and nanoscale in a state-of-the-art cleanroom and associated laboratories. Capabilities include state-of-the art electron-beam lithography, photolithography, thin film deposition, dry etching, back end processing, metrology, materials characterization (Scanning Electron Microscopy (SEM), Focused Ion Beam (FIB), Atomic Force Microscopy (AFM), etc.), wafer dicing, and microfluidics device development (Hot embossing, PDMS processing, micromilling, etc). The facility is open to users from academia, government laboratories and industry.
See training calendar at http://nano3.calit2.net/courses/
|Electron-beam Lithography, Photolithography, Thin film deposition, Dry etching, SEM (Scanning Electron Microscopy), Micromilling, FIB (Focused Ion Beam), Atomic Force Microscopy (AFM), Metrology instruments (various), PDMS (Poly(DiMethylSiloxane) processing|
|CAMPUS||CASS||CASS Research and Engineering Services and Test Facilities||
Research and Engineering Services and Test Facilities: The CASS thermal/vacuum facility is a vacuum chamber (4 ft diameter x 7 ft long) with porting for cold gas/LN2 interface to custom thermal shrouds with heaters for thermal control in the -150C to +50C range. Vacuum pressures of 10-6 Torr are achievable through the diffusion pump and cold trap. Automated control of the thermal system and cold trap nitrogen supply allows users to operate the system around the clock for cost effective thermal/vacuum environmental testing of space instrumentation. CASS engineers and programmers have decades of experience in instrument and system design for radiation detection and space applications. They have developed lung function instrumentation for the astronauts on the Space Shuttle, high resolution electron detectors for electron microscopy at the National Center for Microscopy and Imaging Research, as well as packaging phototubes for use in hostile environments deep undersea or in outer space.
|Vacuum chamber (thermal/vacuum), Radiation Detection instruments|
|CAMPUS||Chemistry & Biochemistry||Biomolecular and Proteomic Mass Spectrometry Facility||
Core services include protein identification from gels or complex mixtures, multidimensional separations prior to nanoLC MSMS, protein quantitation by SILAC and iTRAQ, identification of post-translational modification including phosphorylation, acetylation, and methylation, walk-up MALDI-TOF for trained users to analyze biomolecular samples. Core equipment includes ABSciex 5600 triple-TOF, QSTAR Elite hybrid Q-TOF (AB Sciex), 4800 MALDI-TOFTOF (AB Sciex), Akta Explorer for off-line multidimentional chromatography, and Voyager DE-STR MALDI-TOF (AB Sciex).
|CAMPUS||Chemistry & Biochemistry||Chemistry & Biochemistry Fabrication Facility||
The Fabrication Facility, located in room 1610 of York Hall, is a full service development, renovation, demolition, and machine and wood shop. The Facility provides the following services: Minor Renovations, Painting Projects, Custom Lab and Office Cabinetry and Shelving, Office Relocations, Earthquake Restraints.
|CAMPUS||Chemistry & Biochemistry||Chemistry Nuclear Magnetic Resonance Facility||
The Chemistry NMR facility features a 500MHz, two 400Mhz, and 300 MHz Varian spectrometers and is located in rooms 5140 and 6118 of Pacific Hall. The Biomolecular NMR facility features a Varian 800MHZ, a Bruker 600MHz and a Varian 500MHz and is located in B007 of the Natural Sciences Building. Reservations are made on a day-to-day basis. During normal business hours, sign-up time is limited to 45 minutes on the 300 and the two 400s. Experiments requiring more time should be performed during the evenings or weekends. For reservation limitations on the 500, please visit the 500 scheduling page. Please note that while all of the facility's spectrometers are capable of a standard battery of experiments, for non-standard applications, please identify which spectrometer fits your needs best before reserving time. If your project has time requirements that extend beyond the normal boundaries of what is available on the scheduler or you experience problems with the instruments, please contact us at email@example.com with your request. This will direct your query to everyone involved with maintaining the NMR facility. Core equipment includes Varian VNMRS 800 NMR with 3x Resonance Cold Probe (NSB), Varian VNMRS 500 NMR (NSB), Bruker Avance 600 NMR with 3x Resonance Cryoprobe (NSB), Varian VNMRS 500 NMR with Xsens Cold Probe (Pacific Hall), JEOL ECA 500 NMR (Pacific Hall), Varian Mercury Plus 400 NMR (Pacific Hall), Varian Mercury 400 NMR (Pacific Hall), and a Varian Mercury 300 NMR (Pacific Hall)
|Spectrometer, NMR (Nuclear Magnetic Resonance)|
|CAMPUS||Chemistry & Biochemistry||Chemistry Stockroom||
The Stockroom is located on the first floor of Urey Hall. We offer a variety of laboratory and office products, from special chemicals to ordinary office supplies. In addition, a selection of standard tools and electronics components is available. As a recharge facility, the Stockroom extends its services to groups possesing a valid index number, including any of the academic departments at UCSD, as well as SIO. The Stockroom phone is 858-534-2017
|CAMPUS||Chemistry & Biochemistry||Cryo-Electron Microscope Facility||
The Cryo-Electron Microscopy Facility is available to UCSD faculty, staff and students and also to members of the non-profit research institutions on the Torrey Pines Mesa. The FEI Tecnai G2 Polara is a 300KV transmission electron microscope (TEM) equipped with a field emission gun (FEG). It has a newly developed stage that allows for examination of multiple, vitrified samples at either liquid nitrogen or liquid helium temperatures as well as a state of the art K2 direct detector and energy filter. The FEI Tecnai G2 Sphera is a 200KV TEM equipped with a LaB6 electron gun. It has the standard holder for dry samples and two Gatan cryotransfer holders. The Polara is equipped with the SerialEM tomography software and the Leginon image acquisition system. Both microscopes are available for use on a recharge basis, however use of the Polara will be restricted to those scientists whose projects require the higher resolution afforded by the FEG or the greater stability of the Compustage. The only sample preparation techniques performed in the facility are plunge freezing of particulate samples for cryomicroscopy and negative staining. Operation of the instruments by Facility staff is available as is hands-on training on the instruments.
|TEM (transmission electron microscope)|
|CAMPUS||Chemistry & Biochemistry||Environmental and Complex Analysis Lab (ECAL)||
The Environmental and Complex Analysis Laboratory (ECAL) is a state-of-the-art analytical facility that houses cutting-edge chromatography and mass spectrometry systems through unique partnerships between UCSD and Thermo Scientific. The facility serves as a platform to develop innovative strategies for performing sensitive, accurate and precise analysis of complex environmental samples as well as other samples that may require unique applications in order to provide the highest quality analysis. Novel approaches to providing a comprehensive composition analysis of both complex and environmental samples can be implemented through the following state-of-the-art analytical systems within the ECAL facility: ultra-high resolution hybrid linear ion trap-Orbitrap mass spectrometer (Orbitrap Elite), gas chromatograph coupled to a triple-quadrupole detector (GC-TQD), 2-D and 1-D ultra-performance liquid chromatography (UPLC) systems with UV/Vis detection (all UPLC systems are capable of being connected to the Orbitrap Elite system), inductively couple plasma quadrupole mass spectrometer (ICP-MS), as well as an ion chromatograph with a conductivity detector. The ECAL facility also houses a variety of extraction platforms to aid in preparing complex samples for analysis.
|Mass Spectrometer, Extraction platforms, UPLC (Ultra Performance Liquid Chromatography), GCMS (Gas Chromatography Mass Spectrometry), ICP-MS (Inductively Coupled Plasma Mass Spectrometer)|
|CAMPUS||Chemistry & Biochemistry||Molecular Mass Spectrometry Facility||
The Molecular Mass Spectrometry Facility (MMSF) houses two Thermo LCQdeca-MS, a Thermo LCQdeca XPplus MS, a Micromass Quattro Ultima Triple Quadrupole MS, an Agilent 6230 Accurate-Mass high resolution TOFMS, a Thermo Traceplus GC-MS, a Bruker Biflex IV MALDI-TOFMS, an Agilent 1100 HPLC, and two Agilent 1260 HPLC systems. The goal of the facility is provide collaborative interaction with mass spectrometry experts so that the highest quality analysis can be obtained. The MMSF provides routine MS and MS/MS, LC-MS, LC-MS/MS, HR-MS, LC-HR-MS analysis on various types of samples including synthetic organic molecules, synthetic peptides, natural products, organometallic compounds, intact proteins, and oligonucleotides.
|Mass Spectrometer, HPLC (High Performance Liquid Chromatography)|
|CAMPUS||Institute for Genomic Medicine||IGM Genomics Center||
The IGM Genomics Facility at the University of California, San Diego, is a core research facility overseen by the IGM. Located in Leichtag 172, the IGM Genomics Facility provides a variety of services, including sequencing library preparation and sequencing on Illumina's HiSeq4000, HiSeq2500, and MiSeq platforms as well as on the PacBio RS II Sequencing System. We also process Illumina Infinium Beadchips, which includes a variety of whole-genome genotyping (WGGT) arrays as well as the Infinium HumanMethylation450 BeadChip. For our complete offerings, please visit the Services tab. The mission of the IGM Genomics Facility is to provide UCSD researchers, as well as researchers in the greater UC community, high quality standard, cutting-edge, and custom genomics services as well as consultation on experimental design and training/education about genomic methods. We can also provide our services to researchers at other research institutions and to industry. Services:Study Design Consultation, Sample QC, Covaris Shearing, Next Gen Sequencing Library Prep (RNA/DNA/SmallRNA), Exome Sequencing, Illumina Sequencing Runs, Genotyping Arrays, Methylation Arrays, PacBio SMRT Sequencing