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|Cryo-Electron Microscope Facility||
The Cryo-Electron Microscopy Facility is available to UCSD faculty, staff and students and also to members of the non-profit research institutions on the Torrey Pines Mesa. The FEI Tecnai G2 Polara is a 300KV transmission electron microscope (TEM) equipped with a field emission gun (FEG). It has a newly developed stage that allows for examination of multiple, vitrified samples at either liquid nitrogen or liquid helium temperatures as well as a state of the art K2 direct detector and energy filter. The FEI Tecnai G2 Sphera is a 200KV TEM equipped with a LaB6 electron gun. It has the standard holder for dry samples and two Gatan cryotransfer holders. The Polara is equipped with the SerialEM tomography software and the Leginon image acquisition system. Both microscopes are available for use on a recharge basis, however use of the Polara will be restricted to those scientists whose projects require the higher resolution afforded by the FEG or the greater stability of the Compustage. The only sample preparation techniques performed in the facility are plunge freezing of particulate samples for cryomicroscopy and negative staining. Operation of the instruments by Facility staff is available as is hands-on training on the instruments.
|Environmental and Complex Analysis Lab (ECAL)||
The Environmental and Complex Analysis Laboratory (ECAL) is a state-of-the-art analytical facility that houses cutting-edge chromatography and mass spectrometry systems through unique partnerships between UCSD and Thermo Scientific. The facility serves as a platform to develop innovative strategies for performing sensitive, accurate and precise analysis of complex environmental samples as well as other samples that may require unique applications in order to provide the highest quality analysis. Novel approaches to providing a comprehensive composition analysis of both complex and environmental samples can be implemented through the following state-of-the-art analytical systems within the ECAL facility: ultra-high resolution hybrid linear ion trap-Orbitrap mass spectrometer (Orbitrap Elite), gas chromatograph coupled to a triple-quadrupole detector (GC-TQD), 2-D and 1-D ultra-performance liquid chromatography (UPLC) systems with UV/Vis detection (all UPLC systems are capable of being connected to the Orbitrap Elite system), inductively couple plasma quadrupole mass spectrometer (ICP-MS), as well as an ion chromatograph with a conductivity detector. The ECAL facility also houses a variety of extraction platforms to aid in preparing complex samples for analysis.
|Molecular Mass Spectrometry Facility||
The Molecular Mass Spectrometry Facility (MMSF) houses two Thermo LCQdeca-MS, a Thermo LCQdeca XPplus MS, a Micromass Quattro Ultima Triple Quadrupole MS, an Agilent 6230 Accurate-Mass high resolution TOFMS, a Thermo Traceplus GC-MS, a Bruker Biflex IV MALDI-TOFMS, an Agilent 1100 HPLC, and two Agilent 1260 HPLC systems. The goal of the facility is provide collaborative interaction with mass spectrometry experts so that the highest quality analysis can be obtained. The MMSF provides routine MS and MS/MS, LC-MS, LC-MS/MS, HR-MS, LC-HR-MS analysis on various types of samples including synthetic organic molecules, synthetic peptides, natural products, organometallic compounds, intact proteins, and oligonucleotides.
|IGM Genomics Center||
The IGM Genomics Facility at the University of California, San Diego, is a core research facility overseen by the IGM. Located in Leichtag 172, the IGM Genomics Facility provides a variety of services, including sequencing library preparation and sequencing on Illumina's HiSeq4000, HiSeq2500, and MiSeq platforms as well as on the PacBio RS II Sequencing System. We also process Illumina Infinium Beadchips, which includes a variety of whole-genome genotyping (WGGT) arrays as well as the Infinium HumanMethylation450 BeadChip. For our complete offerings, please visit the Services tab. The mission of the IGM Genomics Facility is to provide UCSD researchers, as well as researchers in the greater UC community, high quality standard, cutting-edge, and custom genomics services as well as consultation on experimental design and training/education about genomic methods. We can also provide our services to researchers at other research institutions and to industry. Services:Study Design Consultation, Sample QC, Covaris Shearing, Next Gen Sequencing Library Prep (RNA/DNA/SmallRNA), Exome Sequencing, Illumina Sequencing Runs, Genotyping Arrays, Methylation Arrays, PacBio SMRT Sequencing
|MAE Dynamic Testing & Characterization Facility||
Specializes in measurement of the dynamic properties of materials. This includes high strain rate testing using both tension and compression split Hopkinson bars as well as ballistic and impact testing.A variety of diagnostics are available including high speed photography as well as flash X-ray and a variety of optical techniques.CEAM has the capability to test a wide variety of materials including ceramics, metals, composites, concrete, and polymers at the appropriate strain rates and temperatures.Strain rates from below 100/sec to more than 30,000/sec are possible, temperatures range from -180 C to 1000 C. In addition to the dynamic facilities, quasi-static testing is also available. Core equipment includes: Split Hopkinson bars from 3 mm diameter to 76 mm diameter in both tension and compression (5 total), 25.4mm inch light gas gun 1 km/sec velocity, 56 mm Light gas gun 1 km/sec velocity, 150 mm Light gas gun 400/sec velocity, Imacon 200 Highspeed framing camera 16 frames maximum frame rate 5 nanosec/frame, HP 150 and 450 KV flash X-rays, Instron 20 Kip load frame, MTS biaxial 5 kip load frame, MTS 20 Kip load frame, MTS 50 kip load frame, Matec TB 1000 Ultrasonic analyzer, and a Ritec GA 2500 based Ultrasonic analyzer.
|NanoEngineering Research Facility||
The proposed research program will utilize the facilities and equipment in the brand new, shared user facility, the NanoEngineering-Materials Research Center (NE-MRC), occupying nearly 9,000 sq.ft. in the SME building, housing more than 100 pieces of equipment and instrumentation specifically for materials research that supports the closing the loop goal. The equipment is divided among 5 large Suites that are interconnected: 1) an Electron Optics and Microanalysis Suite that houses 3 SEMs, two W-gun and the other FEG, two with EDS and EBSD capabilities, a 300kV TEM, and a 200kV FEG-STEM with EDS, 2) an Analytical Instrumentation Suite containing complete array of thermal analysis instruments, 3 XRD units, one with a high temperature stage, UV-VIS-NIR, RAMAN, AFMs, FTIR, and optical microscopes, 3) a Materials Preparation Suite with cutting, mounting, grinding, and polishing equipment plus 4 Chem-Hoods, 4) a Materials Synthesis Suite, with heat treatment furnaces, arc and induction melting, rolling mills, cold isostatic press, a new 2200C Spark Plasma Sintering machine, and a new 2500C vacuum/inert gas hot press and 5) the Mechanical Behavior Suite, which houses 3 servo-hydraulic load frames, 2 screwdriven load frames with high temperature testing capabilities, 2 impact testers, and 2 hardness machines Among the equipment located in this facility are SIO's (Emily Chin) SEM facility and SIO's (Gustaf Arrhenius) X-ray diffractometer.
|San Diego Supercomputer Center||
The San Diego Supercomputer Center, a leader in data-intensive computing and cyberinfrastructure, provides resources, services, and expertise to UC campuses as well as the national research community including academia, industry, and government. Core services available via recharge to UC researchers include high performance research computing and data storage, IT equipment co-location services, grant & proposal support, and advanced user support including scientific and application programming, parallel programming, code optimization, etc. User support staff have domain expertise in astronomy, bioinformatics, computational chemistry, computer science, data mining, engineering, genomics, geophysics, machine learning, physics, simulation, and other areas. High Performance Computing systems range from the large-scale Comet and Gordon systems to the medium-scale Triton Shared Computing Cluster. Many services are available to industry and other external entities via service agreement (commercial contract).
|Integrated Digital Infrastructure||
UCSD's Integrated Digital Infrastructure was developed to improve delivery of digital services, and to help guide UCSD faculty to new high-end IT opportunities. IDI provides a concierge service to guide you to the right services to meet your research and instructional needs; support of critical UCSD IT infrastructure, such as the SDSC colocation facility and the Triton Shared Computing Cluster, to maintain availability and ensure moderate pricing of important basic services; a new Library system for the preservation and sharing of curated UCSD digital assets such as research data sets; and opportunity funds for transformational projects and digital research programs that employ new digital technology in the research lab and in the classroom and lead the way for the UCSD community
|CFAR Genomics and Sequencing Core||
full-service core facility providing services, reagents and expertise to perform assays ranging from small-scale to large-scale analysis of gene expression, microRNA quantitation, virus detection and quantitation, and SNP characterization, using TaqMan and Droplet Digital PCR technologies. We have over 15 years of experience in experimental design, consultation, nucleic acid extraction, cDNA synthesis, primer design, qPCR, and droplet digital PCR. Next generation sequencing for a variety of applications (bacterial genome, genome resequencing, miRNA, other) is available. Core equipment includes: Applied Biosystems 7900HT Fast Real-Time PCR System, Applied Biosystems 7500 Real-Time PCR System, two Bio-Rad QX100 Droplet Digital PCR Systems, Agilent 2100 Bioanalyzer & 2200 Tape Staion, Thermo Scientific NanoDrop ND-1000 and ND-8000 spectrophotometers, Qiagen QIAcube, Life Technologies Ion Torrent Personal Genome Machine and Illumina MiSeq sequencers.
|CORE Bio Services||
Core Bio Services offers high quality biotech products and services at competitive prices to the campus research community and to the San Diego biotech community. Core stocks restriction enzymes, modifying enzymes, transformation and transfection products, growth factors, ultrapure reagents, DNA sequencing and much more from a variety of vendors. Core also provides secure liquid nitrogen storage for cell lines and biologicals.